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We narrowed to 9,048 results for: sgRNA

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Showing: 6641 - 6660 of 9048 results

  1. HCP7

    Plasmid
    #166109
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166109-plasmid-map-sequence-id-330317

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  6. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104912
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    104912-plasmid-map-sequence-id-206066

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  8. pAAV-CMV-Dpi2Cas9-puro

    Plasmid
    #232113
    Purpose
    Expresses Dpi2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232113-plasmid-map-sequence-id-462529

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  9. pAAV-CMV-Slu1Cas9-puro

    Plasmid
    #232111
    Purpose
    Expresses Slu1Cas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232111-plasmid-map-sequence-id-462528

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  10. pAAV-CMV-Slu2Cas9-puro

    Plasmid
    #232112
    Purpose
    Expresses Slu2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232112-plasmid-map-sequence-id-462527

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  11. pAAV-CMV-SeqCas9-puro

    Plasmid
    #232110
    Purpose
    Expresses SeqCas9 and puromycin resistance genes, and cloning backbone for sgRNA
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232110-plasmid-map-sequence-id-462526

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  12. pNSU299_CPR1_2

    Plasmid
    #166071
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic region near CPR1
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166071-plasmid-map-sequence-id-330355

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  15. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid
    #170185
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.
    Depositor
    Hunter Fraser
    Article
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Inserts
    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    170185-plasmid-map-sequence-id-333571

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  16. pUC57-white[coffee]

    Plasmid
    #84006
    Purpose
    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4
    Depositor
    Phillip Zamore
    Article
    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.
    Insert
    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    Use
    Unspecified
    Mutation
    A GC-to-AA mutation that creates a G589E missense…
    Available Since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    84006-plasmid-map-sequence-id-165529

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  17. pEHE51

    Plasmid
    #250522
    Purpose
    Vector for sgRNA expression in Acinetobacter baumannii (CRISPRi). Modified version of pYDE007 (Plasmid #194152) made Golden Gate compatible (BsaI) for sgRNA oligo insertion.
    Depositor
    Ralph Isberg
    Article
    Hamami et al bioRxiv [Preprint]. 2025 Aug 4:2024.01.0
    Insert
    Two BsaI sites (T-BsaI-BfuI-BsaI-A) inserted between the J23119 promoter and dCas9 handle/gRNA scaffold
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    250522-plasmid-map-sequence-id-497877

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Showing: 6641 - 6660 of 9048 results