We narrowed to 9,174 results for: Pol

1. pCAG-mCherry-FKBP-GEF(ARHGEF1)

   Plasmid

   #85152

   Purpose

   acute activation of Rho through rapamycin-induced translocation to membrane-localized FRB

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Fusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630) (ARHGEF1 Human)
   

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHRIG-Akt3

   Plasmid

   #53596

   Purpose

   lentiviral expression of constitutively active human Akt3 and EGFP

   Depositor

   Heng Zhao

   Article

   Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

   Insert

   cAkt3 (AKT3 Human)
   

   Use

   Lentiviral

   Tags

   HA and IRES-EGFP

   Expression

   Mammalian

   Mutation

   constitutively active (myristoylated version)

   Promoter

   CMV

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pGM238

   Plasmid

   #220178

   Purpose

   Expresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3)

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   NACA and BTF3 (NACA, BTF3 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG (NACA); 6xHis (BTF3)

   Mutation

   K78E (NACA) + K43B (BTF3)

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. BCL6-GFP in Artichoke

   Plasmid

   #169931

   Purpose

   Overexpression of Full Length of BCL6-GFP fusion

   Depositor

   Benjamin Ebert

   Article

   Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

   Insert

   BCL6 (BCL6 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. hGFAP-Cre

   Plasmid

   #40591

   Purpose

   Mouse astrocyte expression of Cre

   Depositor

   Albee Messing

   Article

   Zhuo et al Genesis. 2001 Oct;31(2):85-94.

   Insert

   Cre recombinase (GFAP Mouse, Human, Phage)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   MP-1 fragment (mouse protamine 1 intron and polya…

   Expression

   Mammalian

   Mutation

   Construct contains a nuclear-targeted Cre recombi…

   Promoter

   hGFAP promoter

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_DmKHC[1-421]-1xGFP-6xHis

   Plasmid

   #196972

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-N-Myc_535-1102 USP7

   Plasmid

   #131246

   Purpose

   Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Expresses USP7 amino acids 535-1102. This truncat…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-N-Myc_1-205 USP7

   Plasmid

   #131245

   Purpose

   Mammalian expression of the USP7 TRAF-like domain with an N-terminal Myc tag.

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Expresses a mutant form of our pcDNA3.1-N-Myc_1-5…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR-XL-rAkap9

   Plasmid

   #196867

   Purpose

   Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akap9 (Akap9 Rat)
   

   Expression

   Bacterial

   Promoter

   Lac promoter and T7 promoter

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

    Plasmid

    #217801

    Purpose

    Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDECKO_GFP

    Plasmid

    #141210

    Purpose

    empty GFP vector for cloning sgRNA or pgRNAs

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBactin-EB3-mNeonGreen

    Plasmid

    #196848

    Purpose

    labelling of microtubule plus ends

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    EB3-NeonGreen (MAPRE3 Human)
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-Puro-Myr-Flag-IKBKE

    Plasmid

    #15293

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Myr-Flag

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-N-Myc_1-560 USP7

    Plasmid

    #131244

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses USP7 amino acids 1 - 560. This truncati…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid

    #208946

    Purpose

    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-N-Myc_535-888 USP7

    Plasmid

    #131248

    Purpose

    Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

    Plasmid

    #211115

    Purpose

    For tagging the C-term of Top2A with Venus

    Depositor

    Job Dekker

    Article

    Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

    Inserts

    5' C-term Top2A homology arm (TOP2A Human)
    Venus
    T2A
    Blastocidin Resistance
    5' C-term Top2A homology arm (TOP2A Human)
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    n/a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. hu-TLR4 399 SNP flag pLenti4/To/v5/DEST

    Plasmid

    #42651

    Purpose

    destination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 399 SNP (TLR4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T399I

    Promoter

    CMV/TO

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)

    Plasmid

    #217804

    Purpose

    Unfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-linker-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCJ202

    Plasmid

    #162675

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    W159C, S238C; codon optimized for expression in E…

    Promoter

    T7/lac

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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