We narrowed to 26,139 results for: Vit

1. pET28-SaCas9

   Plasmid

   #178901

   Purpose

   SaCas9 (Cas9 from Staphylococcus aureus)

   Depositor

   Hendrik Milting

   Article

   Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

   Insert

   SaCas9
   

   Use

   Tags

   His6-Tag, bipartite nuclear localization signal f…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRN3P-HA-Suv4-20h2

   Plasmid

   #86691

   Purpose

   For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

   Insert

   histone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)
   

   Use

   Tags

   HA

   Expression

   Mutation

   Promoter

   T3

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. piGECI-N1

   Plasmid

   #160421

   Purpose

   Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSynapsin1-GCaMP6s-P2A-mKate2

   Plasmid

   #112006

   Purpose

   For co-expression of GCaMP6s with mKate2

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV9

   Insert

   GCaMP6s-P2A-mKate2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEMHE-mEGFP-mTrim21

   Plasmid

   #105519

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   T7

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. wtBxb1_IVT

   Plasmid

   #222340

   Purpose

   Plasmid for wild type Bxb1 mRNA in vitro transcription template

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized wtBxb1
   

   Use

   Template for in vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7 (inactivated)

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPIK16045_pX330_Flag-SuperFi-Cas9

   Plasmid

   #184370

   Purpose

   Mammalian expression of SuperFi-Cas9 nuclease

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mammalian

   Mutation

   Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…

   Promoter

   CBh

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag Nprl2 pRK5

   Plasmid

   #46333

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   Nprl2 (NPRL2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMAL-PUM1

   Plasmid

   #120384

   Purpose

   Bacterial expression of MBP-Pumillio1 fusion protein

   Depositor

   Igor Ulitsky

   Article

   Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

   Insert

   PUM1 (PUM1 Human)
   

   Use

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221_HUWE1_isoform1

    Plasmid

    #190464

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-Hsp-eGFP-PGK-mCherry

    Plasmid

    #191862

    Purpose

    Heat inducible eGFP reporter with constitutive mCherry marker

    Depositor

    Yingxiao Wang

    Article

    Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

    Inserts

    eGFP
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSPA7 promoter and PGK promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQE-80L MBP-SspB Nano

    Plasmid

    #60409

    Purpose

    E. coli expression of SspB Nano

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    SspB nano
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    Y11K and A15E

    Promoter

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. H1.224.M.luc

    Plasmid

    #193705

    Purpose

    Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated

    Depositor

    Elena Herrera-Carrillo

    Article

    Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

    Insert

    Truncated PolIII H1 promoter (H1.224) with the TATA box mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pILGFPB5A

    Plasmid

    #83556

    Purpose

    Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    GAL1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5 FRT/TO Plk4 ∆24-mCherry

    Plasmid

    #80271

    Purpose

    mammalian expression plasmid for Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DUSP6

    Plasmid

    #27975

    Depositor

    Igor Astsaturov

    Article

    Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIRES2-hPAC

    Plasmid

    #129488

    Purpose

    Expresses human TMEM206

    Depositor

    Zhaozhu Qiu

    Article

    Yang et al Science. 2019 Apr 26;364(6438):395-399.

    Insert

    Transmembrane protein 206 (PACC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EKARdual

    Plasmid

    #99870

    Purpose

    Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

    Depositor

    Marc Tramier

    Article

    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

    Insert

    EKAR
    

    Use

    Tags

    ShadowG and mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEJS2437_Nme2.Smu-ABE8e-nt

    Plasmid

    #201519

    Purpose

    Mammalian expression of N-terminally fused Nme2.Smu-ABE8e-nt

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2.Smu-ABE8e-nt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His-AgdNK

    Plasmid

    #203226

    Purpose

    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    

    Use

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge