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Showing: 6681 - 6700 of 19450 results

  8. pT7-agrBD-IV

    Plasmid
    #53440
    Purpose
    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV
    Depositor
    Cynthia Collins
    Publication
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus (with D29Y mutation in agrD)
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    D to Y mutation of residue 29 of the agrD gene (c…
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53440-plasmid-map-sequence-id-83235

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  11. CCR5-SZ190b-sfGFP

    Plasmid
    #162447
    Purpose
    Expression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentration
    Depositor
    Shuguang Zhang
    Publication
    Qing et al iScience. 2020 Oct 28;23(12):101670. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C-C chemokine receptor type 5 (CCR5 Human)
    Expression
    Mammalian
    Mutation
    truncation from aa88 to aa249
    Promoter
    CMV
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    162447-plasmid-map-sequence-id-331353

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  14. pCALNL-Rheb(S16H)

    Plasmid
    #194204
    Purpose
    Cre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.
    Depositor
    Angelique Bordey
    Publication
    Lin et al Proc Natl Acad Sci U S A. 2016 Oct 4;113
    Insert
    Ras Homolog Enriched Protein in Brain (S16H) (RHEB Human)
    Use
    Cre/Lox
    Expression
    Mammalian
    Mutation
    S16H
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194204-plasmid-map-sequence-id-382462

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  16. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Publication
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

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  17. Intron-Tagging-pX330-Cas9-mCherry

    Plasmid
    #159742
    Purpose
    Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.
    Depositor
    Stefan Kubicek
    Publication
    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    donor plasmid targeting sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159742-plasmid-map-sequence-id-314868

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  20. pTO_Snf7_LAP

    Plasmid
    #101851
    Purpose
    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Publication
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    SNF7 (SNF7 Budding Yeast)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101851-plasmid-map-sequence-id-200251

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Showing: 6681 - 6700 of 19450 results