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pcDNA3-HA-Epsin 2

Plasmid

#22241

Depositor

Pietro De Camilli

Article

Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

Insert

Epsin 2 (EPN2 Human)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
pHDR-eGFP (pXK-1647)

Plasmid

#208824

Purpose

The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR

Depositor

Kun Xu

Article

Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSK267

Plasmid

#129272

Purpose

"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

NLS-VP16-ZF 43-8-NES

Use

Tags

6xHA

Expression

Yeast

Mutation

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBABEhygro-Flag-ZO-2

Plasmid

#27473

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pSLIK TT 3xFLAG ID2 hygro

Plasmid

#98394

Purpose

Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

ID2 (ID2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJEP307-pAAV-EFS(No AgeI)-MCS3-pA

Plasmid

#113684

Purpose

EFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Tags

Expression

Mutation

Promoter

Cytomegalo Virus(CMV)

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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PG13-gal (wt p53 binding sites)

Plasmid

#16446

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_ASCL3_P2A_Hygro_Barcode

Plasmid

#120428

Purpose

Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL3 (ASCL3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG GST DEPDC5

Plasmid

#164983

Purpose

DEPDC5 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

DEPDC5 (DEPDC5 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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prk5-TMEM192-3xHA PM mutant

Plasmid

#149506

Purpose

Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

TMEM192 (TMEM192 Human)

Use

Transient expression

Tags

3XHA

Expression

Mammalian

Mutation

p.LL23-24AA, p.LL28-29AA

Promoter

CMV

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH4330

Plasmid

#191700

Purpose

Punc-17::GtACR2::wCherry unc-54 3' UTR C.elegans neuron expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GtACR2

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Punc-17

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLX302 PCSK5-V5 puro

Plasmid

#98389

Purpose

Lentiviral vector for constitutive expression of human PCSK5 with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pJH4329

Plasmid

#191679

Purpose

Prgef-1-GtACR2::wCherry unc-54 3' UTR C.elegans Pan-neural expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GtACR2

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pregf-1

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV Puro SCRIB P305L

Plasmid

#88887

Purpose

SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

Depositor

Senthil Muthuswamy

Article

Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

Insert

SCRIB (SCRIB Human)

Use

Retroviral

Tags

Expression

Mutation

Proline to Leucine mutation @ 305th amino acid

Promoter

MSCV promoter

Available since

March 2, 2018

Availability

Academic Institutions and Nonprofits only

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CoV2-N-M234I

Plasmid

#177955

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (M234I) (N )

Use

Tags

Expression

Mammalian

Mutation

M234I

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-DNMT3A PWWP

Plasmid

#59696

Purpose

Contains histone modification interacting domain (DNMT3A PWWP)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

DNMT3A PWWP (Dnmt3a Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEb

Plasmid

#193832

Purpose

Express TadCBEb (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDb-SpCas9 D10A-2xUGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_HOXA11_P2A_Hygro_Barcode

Plasmid

#120453

Purpose

Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA11 (HOXA11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-CBX7 Chromo

Plasmid

#59695

Purpose

Contains histone modification interacting domain (CBX7 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

CBX7 Chromo (CBX7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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