We narrowed to 7,895 results for: sms

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pTRE3G-orangutan cGAS-HA

Plasmid

#186871

Purpose

Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Orangutan cGAS with C-terminal HA tag (CGAS Pongo abelii)

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 GST-hUSP27X C87A

Plasmid

#225711

Purpose

Bacterial expression of USP27X (C87A) with GST tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X C87A (USP27X Human)

Tags

GST

Expression

Bacterial

Mutation

C87A, inactivates deubiquitylase

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mOrange/CALR ins5

Plasmid

#214769

Purpose

Mammalian expression of human CALR ins5

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

human CALR ins5 (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.3R/MPL-shRNA4

Plasmid

#204529

Purpose

Lentiviral expression of MPL-shRNA4; gene knock down of human MPL

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-shRNA4 (MPL Human)

Use

Lentiviral

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.3R/MPL-shRNA3

Plasmid

#204528

Purpose

Lentiviral expression of MPL-shRNA3; gene knock down of human MPL

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-shRNA3 (MPL Human)

Use

Lentiviral

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.3R/MPL-shRNA2

Plasmid

#204527

Purpose

Lentiviral expression of MPL-shRNA2; gene knock down of human MPL

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-shRNA2 (MPL Human)

Use

Lentiviral

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.3R/MPL-shRNA1

Plasmid

#204526

Purpose

Lentiviral expression of MPL-shRNA1; gene knock down of human MPL

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-shRNA1 (MPL Human)

Use

Lentiviral

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCASCADE-udhA-xylA

Plasmid

#158612

Purpose

Low phosphate inducible gRNA array to silence the xylA and udhA promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

udhA-xylA guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-gltA2-xylA

Plasmid

#158613

Purpose

Low phosphate inducible gRNA array to silence the xylA and gltA2 promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

gltA2-xylA guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-zwf-xylA

Plasmid

#158614

Purpose

Low phosphate inducible gRNA array to silence the xylA and zwf promoters

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

zwf-xylA guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF3∆165N (H348E)

Plasmid

#175420

Purpose

Encodes His-MBP-TEV-ybbR-H348E eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

∆165N, H348E

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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px330 BRM gRNA

Plasmid

#165586

Purpose

Insertion of BRM degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

BRM (Smarca4 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 NPm

Plasmid

#126593

Purpose

Expresses siRNA resistant SENP2 (nuclear pore mutant) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of amino acids 1-65 and L329A/L331A in t…

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2-R232Q

Plasmid

#124064

Purpose

Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXlATL2-R232Q

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2

Plasmid

#124063

Purpose

Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXIATL2

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5K85R

Plasmid

#98694

Purpose

Gateway cloning of human PRDX5-K85R

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway Cloning

Mutation

K85R

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5K83R

Plasmid

#98693

Purpose

Gateway cloning of human PRDX5-K83R

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway Cloning

Mutation

K83R

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5E80K

Plasmid

#98692

Purpose

Gateway cloning of human PRDX5-E80K

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway Cloning

Mutation

E80K

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSOD1E133insTT-AcGFP1

Plasmid

#83441

Purpose

Expresses SOD1E133insTT in mammalian cells.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

E133insTT

Promoter

CMV

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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1.2 hA33 luciferase

Plasmid

#68149

Purpose

luciferase reporter for hA33 promoter

Depositor

Joan Heath

Article

Johnstone et al J Biol Chem. 2002 Sep 13;277(37):34531-9

Insert

A33 (GPA33 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

starts at -1149 relative to ATG

Promoter

human GPA33 promoter

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only