We narrowed to 11,014 results for: phen
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Plasmid#228627PurposeMammalian expression of mOrange2 and extra insert, for MuSIC barcode constructionDepositorInsertmOrange2
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mRuby3
Plasmid#228628PurposeMammalian expression of mRuby3 and extra insert, for MuSIC barcode constructionDepositorInsertmRuby3
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHsp70(K71E)-GFP
Plasmid#228196Purposeexpressed human Hsp70 (K71E mutation) with a GFP fluorescent protein tagDepositorAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3428 MTK234-LacO-BtoApegRNA
Plasmid#226669PurposeMTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNADepositorInserthU6-LacOS-BtoApegRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhU6-LacOSAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL2670 MTK3-PEmax
Plasmid#226663PurposeMTK3 part containing the coding sequence of PEmaxDepositorInsertPEmax
UseCRISPR and Synthetic BiologyPromoternoneAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-EBFP2-FRB
Plasmid#222417PurposeTo express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)
Plasmid#225430PurposeOCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoterDepositorInsertminiDFR-FRT-OCSterm-FRT-miniDFR
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)
Plasmid#225431PurposeOCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoterDepositorInsertminiDFR-att-OCSterm-att-miniDFR
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaDdCBE 3_CN (right, DddA11-N)
Plasmid#222003PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_CN right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…PromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaDdCBE 3_NC (left, DddA11-N)
Plasmid#222004PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_NC left)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…PromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5418_pMBP-GlcR
Plasmid#221192PurposeExpression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.DepositorInsertglcR (pden4400 from Paracoccus denitrificans)
Tags10x His tag with E. coli maltose binding protein …ExpressionBacterialPromoterT7 promoter and T7 promoter-lacOAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGST-WzzE
Plasmid#196662PurposeExpression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
TagsTEV-cleavable GSTExpressionBacterialMutationValine insertion at position 2.PromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-mCherry-WzzE
Plasmid#196663PurposeExpression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
TagsHis and mCherryExpressionBacterialMutationValine insertion at position 2 of WzzE.PromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPglL-strep-mCherry
Plasmid#196664PurposeExpression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coliDepositorInsertPglL
TagsTEV-cleavable distrep tag II and mCherryExpressionBacterialPromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-LAP2beta
Plasmid#222416PurposeTo express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-BAF
Plasmid#222414PurposeTo express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR051
Plasmid#185029Purpose400 nt l31 promoter region, l31 5'UTR with nt. 47-51 and 80-84 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 47-51 & 80-84 mutated rpmE 5'…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR052
Plasmid#185030Purpose400 nt l31 promoter region, l31 5'UTR with nt. 56-61 and 68-73 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 56-61 and 68-73 mutated in rpmE 5…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR064
Plasmid#185031Purpose400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides of rpmE 5'UTR scrambled, Only fi…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR102
Plasmid#185033Purpose400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationU54C mutation of rpmE 5'UTR, Only first 90 n…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR106
Plasmid#185036Purpose400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationG79U mutation of rpmE 5'UTR, Only first 90 n…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR116
Plasmid#185040Purpose400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationU58C and U70C of rpmE of 5'UTR, Only first 9…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR017-L31-sfGFP
Plasmid#185024Purpose400 nt l31 promoter region, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationOnly first 90 nt of rpmE coding presentAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR039
Plasmid#185025Purpose400 nt l31 promoter region, l31 5'UTR with nt. 1-31 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 1-31 deleted from rpmE 5'UTR, On…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR040
Plasmid#185026Purpose400 nt l31 promoter region, l31 5'UTR with nt. 35-46 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 35-46 deleted from rpmE 5'UTR, O…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR041
Plasmid#185027Purpose400 nt l31 promoter region, l31 5'UTR with nt. 47-54 & 76-86 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 47-54 & 76-86 deleted from rpmE …Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr
Plasmid#218766Purposeencodes EcTyrRS-VSMA* and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-VSMA*
ExpressionBacterialMutationY37V, D167G, D182S, F183M, L186A, D265RPromotertacIAvailable SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-DDX5(kf)-SNAP
Plasmid#166152PurposeExpression of N. furzeri DDX5 in E. coliDepositorInsertDDX5
TagsHis10 and SNAPExpressionBacterialPromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA_F164R238
Plasmid#216230PurposeS. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged arginine 238 to serine, phenylalanine 168…PromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG4 CTF-FLAG
Plasmid#217702PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG4 (ADGRG4 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2721Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only