We narrowed to 20,307 results for: ACE;

1. SLC39A14-GFP

   Plasmid

   #104380

   Purpose

   Expresses SLC39A14/ZIP14 wild-type in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Liu et al J Biol Chem. 2017 Oct 6;292(40):16605-16

   Insert

   SLC39A14 (SLC39A14 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR-cBEST-v2-PermE

   Plasmid

   #209446

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; sgRNA expression from ermE* promoter

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3-EMTB-TurboID-V5

   Plasmid

   #190736

   Purpose

   Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   ensconsin (MAP7 Human)
   

   Use

   Tags

   Myc, TurboID, and V5

   Expression

   Mammalian

   Mutation

   Microtubule binding-domain of ensconsin (amino ac…

   Promoter

   CMV

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 766 p3E-2A-nlsmCherrypA

   Plasmid

   #195972

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Christian Mosimann, Kristen Kwan

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-nlsmCherry (nuclear)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SaBE4-Gam

   Plasmid

   #100809

   Purpose

   Expresses SaBE4-Gam in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

   Insert

   SaBE4-Gam
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK0_062

   Plasmid

   #123951

   Purpose

   Encodes the 2nd gen. Lenti Virus GFP dropout destination vector without insulator as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pHR DEST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6 SgRNA RBM20

   Plasmid

   #162735

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   Spacer sequence for RBM20
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. (189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)

   Plasmid

   #160739

   Purpose

   Expresses a truncated OGT (4.5 TPRs) fused to a GFP nanobody

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nGFP-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-moxGFP-Puro-H2BC11

   Plasmid

   #207758

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pSN001 pME minprom_mCerulean

    Plasmid

    #195959

    Purpose

    Gateway pME fluorophore

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mouse beta-globin minimal promoter mCerulean
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-O-GECO1

    Plasmid

    #46025

    Purpose

    Orange intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    O-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…

    Promoter

    CMV

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCK002 pME mCerulean

    Plasmid

    #195956

    Purpose

    Gateway pME fluorophore

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mCerulean
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-PITCh-CLTC

    Plasmid

    #227312

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CLTC (CLTC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. HiBiT-FZD7

    Plasmid

    #195845

    Purpose

    Expresses human FZD7 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD7 (FZD7 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGV15 (CK2alpha K68M)

    Plasmid

    #27089

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha K68M (kinase dead) (CSNK2A1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K68M (kinase dead)

    Promoter

    CMV

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

16. GFP-itis pBE-t

    Plasmid

    #195342

    Purpose

    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Inserts

    ecTadA(8e)-SpCas9-NG
    gRNA ctctacgcgggtcttgtagt
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

    Promoter

    Lac and ProC

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEM-Cas9HF1-indRecA56

    Plasmid

    #102294

    Purpose

    Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Inserts

    Cas9HF1
    recA56
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-itis pBE-nt

    Plasmid

    #195343

    Purpose

    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Inserts

    ecTadA(8e)-SpCas9-NG
    gRNA gtgcacgacgccgtatgcga
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

    Promoter

    Lac and ProC

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. meffBlue chromoprotein

    Plasmid

    #117845

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, meffBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-C-miRFP670nano3-Blast-H2BC11

    Plasmid

    #207762

    Purpose

    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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