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Plasmid#207621PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4/5FTrp)RS
Plasmid#207620PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mWatermelon
Plasmid#190625PurposeBacterial expression of mWatermelon GFPDepositorInsertmWatermelon
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAS1NB c Rosella I
Plasmid#71245Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.DepositorInsertRosella
UseTagsExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NP-V5-B/BRISBANE/60/2008
Plasmid#187923PurposeExpresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cellsDepositorInsertInfluenza B Nucleoprotein
UseTagsV5ExpressionMammalianMutationPromoterCMV PromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHT-PIP2
Plasmid#60937PurposeFluorescent FPX sensor for PIP2DepositorInsertPIP2 dimerization dependent sensor RA+B monomers
UseTagsdimerization dependent fluorescent protein monome…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
mVenus-hCOL1A1
Plasmid#140111PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
UseTagsmVenusExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sirius-mT-Sapphire_pcDNA3
Plasmid#89584PurposeGenetically encoded ratiometric fluorescent thermometerDepositorInsertSirius-mT-Sapphire
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-IRES-eGFP
Plasmid#80343PurposeExpresses smURFP and eGFP in mammalian cells.DepositorInsertssmURFP
eGFP
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDSW1728
Plasmid#120812PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)DepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NP-B/BRISBANE/60/2008
Plasmid#187921PurposeExpresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cellsDepositorInsertInfluenza B Nucleoprotein
UseTagsUNTAGGEDExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
mApple-hCOL1A1
Plasmid#140113PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
UseTagsmAppleExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb
Plasmid#182009PurposeCMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell linesDepositorInsertIgKchL_HA_STf_myc_PDGFRtmb
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 iTEV
Plasmid#64277PurposeExpresses infrared fluorescent tobacco etch virus protease sensor (iTEV)DepositorInsertInfrared fluorescent tobacco etch virus protease sensor (iTEV)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
TurboRFP-pBAD
Plasmid#54858PurposeLocalization: Protein Expression Vector , Excitation: 553, Emission: 574DepositorTypeEmpty backboneUseTags6xHis and TurboRFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1_LifeAct-iBlueberry
Plasmid#73176PurposeExpressing iBlueberry and mCherry in mammalian cellsDepositorInsertLifeAct-iBlueberry
UseTagsExpressionMammalianMutationmutation E177Q in iBlueberryPromoterAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_NES_STf
Plasmid#182001PurposeCMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell linesDepositorInsertNES-STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-C-Src-7
Plasmid#55472PurposeLocalization: Membrane/Vesicles, Excitation: 462, Emission: 492DepositorInsertC-Src
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-hCOL1A1
Plasmid#140112PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
UseTagsmCeruleanExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only