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Plasmid#209540PurposeBTK Type 1 homology arm for Golden Gate assemblyDepositorInsertUpstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBR_attB(bxb)_ccdB_lox_v2
Plasmid#198040PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0245
Plasmid#162284PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0285
Plasmid#162290PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract SystemDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0284
Plasmid#162289PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract SystemDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CB0026
Plasmid#162285PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract SystemDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CB0246
Plasmid#162286PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract SystemDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPMY1CB0001
Plasmid#162317PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0283
Plasmid#162288PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract SystemDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0282
Plasmid#162287PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract SystemDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0025
Plasmid#162283PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ142
Plasmid#69294PurposeGolden Gate recipient and Gateway entry vector; assembly of 2 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA_expression_vector
Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ143
Plasmid#69295PurposeGolden Gate recipient and Gateway entry vector; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ144
Plasmid#69296PurposeGolden Gate recipient and Gateway entry vector; assembly of 4 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-gRNA_BbsI_CAM
Plasmid#183222PurposeLbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertLbCas12a guide RNA containing BbsI sites in spacer sequence
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ146
Plasmid#69298PurposeGolden Gate recipient and Gateway entry vector; assembly of 6 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-Destination
Plasmid#37184PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.DepositorTypeEmpty backboneUseTagsFokI nucleaseExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination
Plasmid#40132PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).DepositorTypeEmpty backboneUseTagsFokI nuclease (ELD)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination
Plasmid#40131PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).DepositorTypeEmpty backboneUseTagsFokI nuclease (KKR)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only