We narrowed to 842 results for: golden gate assembly

1. pKL1-422

   Plasmid

   #219410

   Purpose

   Reporter gene (1 operator) for zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   (42-10 op)x1-minCyc1-Venus-tCyc1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKL1-424

   Plasmid

   #219412

   Purpose

   Reporter gene (3 operator) for zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   (42-10 op)x3-minCyc1-Venus-tCyc1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKL1-095

   Plasmid

   #219407

   Purpose

   Reporter gene (4 operators) for zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   (13-6 op)x4-minCyc1-Venus-tCyc1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKL1-423

   Plasmid

   #219411

   Purpose

   Reporter gene (2 operator) for zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   (42-10 op)x2-minCyc1-Venus-tCyc1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGGAselect

   Plasmid

   #195714

   Purpose

   pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pBTK402

   Plasmid

   #110598

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBTK403

   Plasmid

   #110599

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBTK401

   Plasmid

   #110597

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMiNGo11-3

   Plasmid

   #126058

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   mitochondria Rieske iron sulphur protein (1-100 aa)
   ccdB cassette (BsaI)
   GFP11x3 (5 aa linker)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMiNGo11-7

    Plasmid

    #126057

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVP089

    Plasmid

    #222040

    Purpose

    MultiGreen 1.0 base HG entry vector.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    H and G overhangs for GreenGate assembly

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pVP091

    Plasmid

    #222042

    Purpose

    MultiGreen 1.0 base FH entry vector.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    F and H overhangs for GreenGate assembly

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pJB53

    Plasmid

    #226309

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with V(T/S)G to AAA CsoS2 MR1-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKL2-160

    Plasmid

    #219414

    Purpose

    Estradiol-inducible expression of 13-6 Zinc finger activator

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-WT)-PDZLigWT-tSSA1}
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTALYM4

    Plasmid

    #47875

    Purpose

    Destination plasmid with mRuby2 for Golden Gate reaction of TALE assembly

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Type

    Empty backbone

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBTK1056

    Plasmid

    #209546

    Purpose

    BTK Type 4 antibiotic resistance cassette for Golden Gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al bioRxiv 2023.09.19.558440

    Insert

    Type 4 Kanamycin resistance cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pTALYM9

    Plasmid

    #47876

    Purpose

    Destination plasmid with 3xTy1 tags for Golden Gate reaction of TALE assembly

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Ty1tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBTK1052

    Plasmid

    #209542

    Purpose

    BTK Type 2 antibiotic resistance cassette for Golden Gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al bioRxiv 2023.09.19.558440

    Insert

    Type 2 Kanamycin resistance cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57-mini v2-HF24_F4

    Plasmid

    #195718

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#4 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pUC57-mini v2-HF24_F20

    Plasmid

    #195734

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#20 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge