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pT2-CAG-fGFP

Plasmid

#108714

Purpose

Transposon donor plasmid for long-term expression of membrane targetted GFP

Depositor

Madeline Lancaster

Article

Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

Insert

EGFP

Tags

6xHis and farnesylation signal

Expression

Mammalian

Promoter

CAG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pJL057

Plasmid

#177169

Purpose

Expresses a nuclease dead MRE11 in human cells

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

MRE11 H129N (MRE11 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

nuclease-inactivating mutation (H129N)

Promoter

EF1a

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Kozak-rhTRIM5alpha-PFOS

Plasmid

#79038

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

rhesus TRIM5alha-PFOS

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCI_AFF-1-FLAG_H2B-RFP

Plasmid

#240506

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Insert

AFF-1

Tags

H2B-RFP

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_EV-D68_VP1

Plasmid

#202072

Purpose

Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

EV-D68 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET16b-Trx CC32/35SS

Plasmid

#36946

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin 1 (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

both active site cysteines (32,35) mutated to ser…

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pYFAC-CH4

Plasmid

#168980

Purpose

AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in asp…

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172505

Purpose

Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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MSP977

Plasmid

#65774

Purpose

Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135E mutation in Cas9

Promoter

CMV & T7

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b/bPanK

Plasmid

#73619

Purpose

Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

Depositor

Suzanne Jackowski, T. J. Kappock

Article

Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

Insert

pantothenate kinase (coaA Escherichia coli)

Tags

His6

Expression

Bacterial

Mutation

relative to MG1655 sequence, changes glutamate-5 …

Promoter

T7

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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