We narrowed to 12,719 results for: CAR

1. pET28a-hDcp2 E147Q, E148Q

   Plasmid

   #72215

   Purpose

   bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E147Q, E148Q

   Promoter

   T7

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pSMP-SETDB1_1

   Plasmid

   #36347

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   SETDB1 (SETDB1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pDest27-mBIN1+17

   Plasmid

   #61096

   Purpose

   Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells

   Depositor

   Robin Shaw

   Article

   Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

   Insert

   mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCD442

   Plasmid

   #153024

   Purpose

   dCas9, MCP-SoxS(R93A/S101A)

   Depositor

   Jesse Zalatan

   Article

   Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

   Insert

   dCas9, MCP-SoxS(R93A/S101A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   Available since

   Aug. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE2-TetOminiCMV-Oct4

   Plasmid

   #136611

   Purpose

   Dox-inducible lentiviral vector expressing mouse Oct4

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. KG371-GFP-mios

   Plasmid

   #87052

   Purpose

   mammalian expression of mios

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   mios (MIOS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108849

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   nMag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPPC001

   Plasmid

   #171138

   Purpose

   For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   Sp.pCas9, BBa_J23107

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMKO.1 puro-luc (shRNA)

   Plasmid

   #153962

   Purpose

   Expresses a shRNA against firefly luciferase

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   shRNA against firefly luciferase (19-bp-long target)
   

   Use

   Luciferase, RNAi, and Retroviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. -962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic

    Plasmid

    #32737

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pDONR-SUMO1

    Plasmid

    #114388

    Purpose

    pDONR vector with SUMO1 gene

    Depositor

    Vincent Dion

    Article

    Aeschbach et al Sci Rep. 2017 Dec 21;7(1):18026. doi: 10

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q29H compared with NCBI reference NP_003343.1

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFPm-DAD M1041A

    Plasmid

    #25411

    Depositor

    Arthur Alberts

    Article

    Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

    Insert

    Diap3 (Diaph3 Mouse)
    

    Use

    Tags

    EGFP, His, and Myc

    Expression

    Mammalian

    Mutation

    Alanine substitution at critical residue in DAD t…

    Promoter

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4

    Plasmid

    #123658

    Purpose

    DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.

    Depositor

    Stanley Qi

    Article

    Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGW1-Myc-DJ1-L166P/K130R

    Plasmid

    #29349

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Leu166Pro and Lys130Arg

    Promoter

    Available since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCML 961

    Plasmid

    #98848

    Purpose

    pTriFC_aidB. Expresses protein-NYFP and 2MS2BD-5' UTR mStrawberry fusions in E. coli for Dual-fluorescence TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Sowa et al Nucleic Acids Res. 2017 Feb 28;45(4):167

    Inserts

    5' UTR + first 100 nucleotides of coding sequence of the aidB gene
    csrA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacO

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/GS-DJ1-L166P-V5

    Plasmid

    #29343

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    His6 and V5 epitope

    Expression

    Mammalian

    Mutation

    L166P

    Promoter

    Available since

    March 7, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pAcrF2

    Plasmid

    #89234

    Purpose

    Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Insert

    AcrF2
    

    Use

    Tags

    6xHis-TEV

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSMP-EED_2

    Plasmid

    #36386

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EED (EED Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MMTV-VEGF

    Plasmid

    #44625

    Depositor

    Robert Oshima

    Article

    Oshima et al Cancer Res. 2004 Jan 1;64(1):169-79.

    Insert

    VEGF121 (Vegfa Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    contains aa160-368; G165E mutation

    Promoter

    Mouse mammary tumor virus promoter

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pHR RPS27A K113R-3xFlag_IRES-GFP

    Plasmid

    #198391

    Purpose

    RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site mutant (K113R)

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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