We narrowed to 9,174 results for: Pol

1. PB-PARP1-Apex2–eGFP

   Plasmid

   #211555

   Purpose

   A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   Apex2-eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2_RB1#2

   Plasmid

   #174151

   Purpose

   Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   RB1 (RB1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

   Plasmid

   #67985

   Purpose

   Cas9 activity reporter (control) with mCherry and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherryABFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPRv2_AMBRA1#2

   Plasmid

   #174153

   Purpose

   Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA (AMBRA1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL Neo Myr Flag PKM2

   Plasmid

   #20585

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   PKM2 (PKM Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

   Plasmid

   #216479

   Purpose

   lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   wtYAP (YAP1) (YAP1 Human)
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Promoter

   EF1aL and UBC

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.3-Nm-dCas9-p300 Core

   Plasmid

   #61365

   Purpose

   encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   Nm-dCas9-p300 Core (EP300 Human, Neisseria meningiditis, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D16A, D587A, H588A, N611A in Nm Cas9

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-hCCND1-T2A-hCCND2-P2A-hCCND3

   Plasmid

   #174156

   Purpose

   Lentiviral vector expressing all three D-type cyclins (cyclin D1, cyclin D2, cyclin D3) from a single promoter

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   CCND1>T2A>CCND2>P2A>CCND3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZL Neo Myr Flag PDK1

   Plasmid

   #20564

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   PDK1 (PDK1 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZL Neo Myr Flag PTK2

    Plasmid

    #20610

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-Htt171-66Q-myc-WPRE

    Plasmid

    #107934

    Purpose

    Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

    Depositor

    Johan Jakobsson

    Article

    Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

    Insert

    Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)
    

    Use

    AAV

    Promoter

    hSynapsin

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZL Neo Myr Flag PKN2

    Plasmid

    #20587

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PKN2 (PKN2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pLenti HsATP13A2 D508N

    Plasmid

    #171820

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP13A2 D508N

    Depositor

    Veerle Baekelandt

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 (ATP13A2 Human)
    

    Use

    Lentiviral

    Mutation

    WT, D962N

    Promoter

    CMV

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWZL Neo Myr Flag PRKAA1

    Plasmid

    #20595

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PRKAA1 (PRKAA1 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pWZL Neo Myr Flag HK2

    Plasmid

    #20501

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    HK2 (HK2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. Human 3' HP1a AID GFP PuroR

    Plasmid

    #127906

    Purpose

    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    HP1 (CBX5 Mustard Weed, Human)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Mutation

    Inserting GFP AID 2A Puro into mouse 3' Hp1a

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid

    #100936

    Purpose

    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

    Plasmid

    #67975

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFBOH-MHL.RBBP4

    Plasmid

    #125173

    Purpose

    Expresses human RBBP4 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Histone-binding protein RBBP4 (RBBP4 Human)
    

    Tags

    His-TEV site

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUW-SOK

    Plasmid

    #20327

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Sox2-F2A-Oct4-T2A-Klf4 (Sox2, Pou5f1, Klf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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