We narrowed to 10,132 results for: yeast

1. pAG426-GAL-3xFLAG-PolyC

   Plasmid

   #181729

   Purpose

   Galactose inducible expression of 3xFLAG-PolyC

   Depositor

   Alejandro Chavez

   Insert

   3xFLAG-PolyC
   

   Tags

   3xFLAG

   Expression

   Yeast

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBHM2643

   Plasmid

   #229527

   Purpose

   Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Tags

   t2a-mNeonGreen-mAID_opt

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS313_MSN5-LY00018

   Plasmid

   #232903

   Purpose

   Plasmid carrying MSN5 from LY00018 (YJM975) with its native promoter and terminator.

   Depositor

   Jolanda Van Leeuwen

   Insert

   MSN5 (MSN5 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   G1563A

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUSE-URA-Sec7-mGold

   Plasmid

   #218967

   Purpose

   Gene replacement plasmid to label S. cerevisiae Sec7 with mGold

   Depositor

   Benjamin Glick

   Insert

   SEC7-mGold (SEC7 Budding Yeast)
   

   Use

   Gene replacement vector for yeast

   Tags

   mGold

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHflu1

   Plasmid

   #203878

   Purpose

   pSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transfer

   Depositor

   Bogumil Karas

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. KBB258

   Plasmid

   #185072

   Purpose

   Bacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusion

   Depositor

   Ken Belanger

   Insert

   NUP1
   

   Tags

   GST

   Expression

   Yeast

   Mutation

   Nup1 N-term+FXFG repeats

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. HCP2

   Plasmid

   #166104

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. HCP3

   Plasmid

   #166105

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. HCP7

   Plasmid

   #166109

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.

   Depositor

   Matthias Heinemann

   Insert

   Whi5-sg106 (WHI5 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. brr2-C520R pRS313

    Plasmid

    #111412

    Purpose

    brr2-C520R (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    C520R aa mutation

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. brr2-N1104A pRS313

    Plasmid

    #111414

    Purpose

    brr2-N1104A (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    N1104A aa mutation

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. brr2-R1107L pRS313

    Plasmid

    #111416

    Purpose

    brr2-R1107L (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    R1107L aa mutation

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pREP81xV5_ccdB

    Plasmid

    #79019

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pREP41xV5_ccdB

    Plasmid

    #79017

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pREP82xV5_ccdB

    Plasmid

    #79020

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pREP42xV5_ccdB

    Plasmid

    #79018

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK275

    Plasmid

    #73363

    Purpose

    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)

    Depositor

    T. J. Kappock

    Insert

    delta-8 sphingolipid desaturase
    

    Expression

    Yeast

    Mutation

    missing residues 1-6

    Promoter

    GAL1

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. brr2-Q904E pRS313

    Plasmid

    #111413

    Purpose

    brr2-Q904E (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    Q904E aa mutation

    Availability

    Academic Institutions and Nonprofits only

    

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19. brr2-R1107A pRS313

    Plasmid

    #111415

    Purpose

    brr2-R1107A (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    R1107A aa mutation

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid

    #177209

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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