We narrowed to 58,534 results for: ran

1. pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

   Plasmid

   #113884

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   pHR-CMV-TetO2

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAS_4xMma PylT sfGPF 150TAG

   Plasmid

   #154766

   Purpose

   plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG in GFP reporter, U25C in PylT

   Promoter

   EF1

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAS134

   Plasmid

   #211505

   Purpose

   AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

   Depositor

   Randall Platt

   Article

   Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

   Insert

   Venus
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHTNW

   Plasmid

   #136403

   Purpose

   Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

   Plasmid

   #113885

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3 deltaN89 Beta-catenin

   Plasmid

   #19288

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-terminal truncation, deleted aa 1-89

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

7. aeBlue chromoprotein

   Plasmid

   #117846

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, aeBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

   Plasmid

   #203228

   Purpose

   Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Insert

   Membrane-localized optopatch for neuronal dendrites
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-DDX3

   Plasmid

   #44975

   Depositor

   Robin Reed

   Article

   Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

   Insert

   DDX3 (DDX3X Human)
   

   Tags

   HA and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK5-HA-SLC38A9.1

    Plasmid

    #71869

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLacO-pEF1α-RFP

    Plasmid

    #179507

    Purpose

    Expresses monomeric RFP in mammalian cells under a EF1α promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    monomeric RFP
    

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. tet-pLKO shGATA6 puro

    Plasmid

    #72615

    Purpose

    Expresses an inducible short hairpin targeting human GATA6 sequence

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    short hairpin GATA binding protein 6
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Promoter

    TRE Tight

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpCas9_T2A_mCherry sgAAVS1

    Plasmid

    #192480

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HIV-1-GFP ΔEnv

    Plasmid

    #217437

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTmpl-miniDonor-GFP

    Plasmid

    #238107

    Purpose

    Mini donor expressing GFP, used for PCR to generate IVT template.

    Depositor

    Feng Zhang

    Article

    Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

    Insert

    CMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence
    

    Expression

    Mammalian

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid

    #108244

    Purpose

    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    

    Use

    Synthetic Biology

    Promoter

    pLacO1

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-SAM

    Plasmid

    #102559

    Purpose

    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Inserts

    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9 and N55K in MS2

    Promoter

    CAG and EF1A

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. amajLime chromoprotein

    Plasmid

    #117843

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amajLime
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)mGAT1-0-GFP

    Plasmid

    #41662

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C-terminal hydrophobic isoleucine residue added a…

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge