We narrowed to 13,166 results for: OVA
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Plasmid#20325PurposeLentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for iPS cell generationDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only
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pRK5-mEGFP-PHOX2B-WT
Plasmid#194576PurposeMammalian Expression of mEGFP-PHOX2B WT Fusion ProteinDepositorInsertPHOX2B (PHOX2B Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-PHOX2B-MUT
Plasmid#194577PurposeMammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion ProteinDepositorInsertPHOX2B (PHOX2B Human)
UseTagsmEGFPExpressionMammalianMutationS207Afs*102PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2-shATRX-GFP
Plasmid#124259PurposeExpresses shRNA targeting ATRX with a GFP reporter which is driven by the SV40 promoter. Construct has inverted repeats to be used in Sleeping beauty system.DepositorInsertshATRX
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-45: TTN-mEGFP
Plasmid#114412PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassetteDepositorInsertTTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)
Plasmid#174516PurposeRecoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.DepositorInsertsPylS_1R26 - CBZK
Af_TyrRS - p-I-Phe
Ma tRNA pylT(8) with CGA anticodon
A.fulgidus tRNA with CUA anticodon
UseTagsExpressionBacterialMutationCUA anticodon, M.alvus Pyl tRNA with CGA anticodo…PromoterGlnS and lppAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCag SE (Self-excising) FlpO-2A-Cre EV
Plasmid#130986Purposeepisomal expression of FlpO and Cre recombinases, self excisingDepositorInsertFlpO-2A-Cre
UseCre/Lox and Unspecified; Episomal expression vect…TagsExpressionMammalianMutationprotamine intron added to FlpO to prevent bacteri…PromoterCMV/Chick β-actin (CAG)Available sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE
Plasmid#179459PurposeDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)DepositorHas ServiceAAV1InsertJEDI-2P-Kv
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_N-DYK_MTHFD1-NLS
Plasmid#133023PurposeNLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)DepositorInsertMTHFD1 (MTHFD1 Human)
UseTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-RAX-WT
Plasmid#194572PurposeMammalian Expression of mEGFP-RAX WT Fusion ProteinDepositorInsertRAX (RAX Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
JDW 742 (pDONR221-EGFP-KRAS4B-G12V)
Plasmid#156403PurposeGateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codonDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseGateway cloningTagsEGFPExpressionMutationmutant KRAS4B-G12VPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD051-sgMLH1-1
Plasmid#125776Purposeconstitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9DepositorInsertsgMLH1-1 (MLH1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD051-sgMLH1-2
Plasmid#125777Purposeconstitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9DepositorInsertsgMLH1-2 (MLH1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)
Plasmid#156410PurposepCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12VDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseTagsmScarlet-IExpressionMammalianMutationmutant KRAS4A-G12VPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)
Plasmid#156409PurposepCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12DDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseTagsmTagBFP2ExpressionMammalianMutationmutant KRAS4A-G12DPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)
Plasmid#156407PurposepCS2 CMV expression vector driving EGFP fused human KRAS4B-G12VDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseTagsEGFPExpressionMammalianMutationmutant KRAS4B-G12VPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-SD1-B.1.1.529-AVI
Plasmid#181704PurposeExpresses SARS-CoV-2 RBD-SD1 domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-B.1.1.529 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationG339D, S371L, S373P, S375F, K417N, N440K, G446S, …PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)
Plasmid#156415Purposekdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseZebrafish transgenesisTagsmTagBFP2ExpressionMutationmutant KRAS4A-G12DPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-RAX-MUT
Plasmid#194573PurposeMammalian Expression of mEGFP-RAX Mutant (S222Rfs*63) Fusion ProteinDepositorInsertRAX (RAX Human)
UseTagsmEGFPExpressionMammalianMutationS222Rfs*63PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-MYOD1-MUT
Plasmid#194571PurposeMammalian Expression of mEGFP-MYOD1 Mutant (R188Pfs*90) Fusion ProteinDepositorInsertMYOD1 (MYOD1 Human)
UseTagsmEGFPExpressionMammalianMutationR188Pfs*90PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_N-DYK_MTHFD1-NES
Plasmid#133022PurposeNES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)DepositorInsertMTHFD1 (MTHFD1 Human)
UseTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD(aa330-526)-B.1.1.529-AVI
Plasmid#182930PurposeExpresses SARS-CoV-2 RBD (aa324-526) domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD(aa324-526)-B.1.1.529 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationG339D, S371L, S373P, S375F, K417N, N440K, G446S, …PromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD(aa335-526)-B.1.1.529-AVI
Plasmid#181701PurposeExpresses SARS-CoV-2 RBD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-B.1.1.529 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationG339D, S371L, S373P, S375F, K417N, N440K, G446S, …PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor TagBFP2-3XFlag (cyto) WPRE
Plasmid#129421PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter proteinDepositorInsertTagBFP2-3Flag
UseMosaic analysis for dual recombinase-mediated cas…Tags3X Flag epitope tagExpressionMutationPromoternone (3X polyA to mitigate episomal expression)Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLBCX-Large-T-antigenK1 mutant
Plasmid#189978PurposeRetroviral vector used to immortalize human cellsDepositorInsertLarge-T-antigenK1 mutant
UseTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)
Plasmid#156416Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseZebrafish transgenesisTagsmScarlet-IExpressionMutationmutant KRAS4A-G12VPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 PSMB5 guide 1
Plasmid#117073Purposesingle guide RNA targeting PSMB5; guide 1DepositorInsertLMPX (PSMB5 Human)
UseCRISPRTagsExpressionMutationPromoterhU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003-sgWRN-EIJ
Plasmid#125774Purposeconstitutive expression of a guide RNA targeting an exon-intron junction of human WRNDepositorInsertsgWRN-EIJ (WRN Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
KRAS-A146T
Plasmid#154426PurposeA146T Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-NTD-B.1.1.529-AVI
Plasmid#181698PurposeExpresses SARS-CoV-2 NTD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-B.1.1.529 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationA67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only