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EGFP-Mm_Plk5(D168A)

Plasmid

#136318

Purpose

Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (Plk5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

D168A

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Hu_Plk5(W207F)

Plasmid

#136320

Purpose

Mammalian expression of human Plk5 W207F with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (PLK5 Human)

Use

Tags

Expression

Mammalian

Mutation

W207F

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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opto-E-cad GFP

Plasmid

#203327

Purpose

Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

Depositor

Seraphine Wegner

Article

Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

Insert

Photoswitchable - E-cadherin with GFP tag (CDH1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Addition of Aslov2 domain at T133 of human E-cadh…

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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mouse E-cadherin GFP (423)

Plasmid

#67937

Purpose

Mammalian expression of E-cadherin-GFP

Depositor

Alpha Yap

Article

Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

Insert

E-cadherin (Cdh1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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proE-cad178-Luc-mEbox

Plasmid

#42082

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -178 to …

Promoter

E-cadherin

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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Murine E-cadherin mCherry

Plasmid

#71366

Purpose

mammalian expression of murine E-caderin with mCherry fusion

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

E-cadherin (Cdh1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-E-cadherin-RFP

Plasmid

#79603

Purpose

pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

CDH1 promoter region (CDH1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pSEVA228-pro4IUPi

Plasmid

#122018

Purpose

Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

Depositor

Gregory Stephanopoulos

Article

Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

Inserts

Choline kinase (CKI1 Budding Yeast)
Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
isopentenyl-diphosphate Delta-isomerase

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pro4

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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7TFP CDH1 mutant reporter

Plasmid

#91703

Purpose

lentiviral luciferase reporter containing CDH1 promoter with mutant E-boxes

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

CDH1 mutant promoter (CDH1 Human)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutant E-boxes

Promoter

CDH1 mutant

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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IL2R/hE-cadherin-cytotail

Plasmid

#45773

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Interleukin-2 receptor α subunit extracellular an…

Promoter

CMV

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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hE-cadherin/α-catenin fusion-pcDNA3

Plasmid

#45771

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Xe α-catenin fusion (CDH1, ctnna1 Frog, Human)

Use

Tags

Expression

Mammalian

Mutation

Human E-cadherin (aa 1-801) fused to Xenopus α-ca…

Promoter

CMV

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
pGL3-E-cadherin promoter

Plasmid

#61798

Purpose

Luciferase reporter containing the mouse E-cadherin promoter

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

-178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
proE-cad670-Luc-mEbox

Plasmid

#42084

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -670 to …

Promoter

E-cadherin

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMIR-CDH1 wtUTR

Plasmid

#25038

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 3'UTR (CDH1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
ArpC3-FLAG_pLib

Plasmid

#173680

Purpose

ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC3 (ARPC3 Human)

Use

Tags

TEV cleavage site and FLAG tag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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ArpC2_pLib

Plasmid

#173679

Purpose

ArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC2 (ARPC2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pmiR-report-hARPC2

Plasmid

#112549

Purpose

3'UTR reporter assay

Depositor

Shusheng Wang

Article

Li et al Sci Rep. 2017 Apr 19;7(1):915. doi: 10.1

Insert

ARPC2 (ARPC2 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF2029

Plasmid

#143566

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMARCA5 (SMARCA5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-z ONE-GO

Plasmid

#189734

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-z ONE-GO
GNAZ (Gnaz Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-oA ONE-GO

Plasmid

#189732

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-oA ONE-GO
GNAO (Gnao1 Rat)

Use

Lentiviral

Tags

Expression

Mutation

I250 (silent mutation in YFP)

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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