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Plasmid#49001Purposefull length coding sequence for zebrafish fli1a gene in gateway middle entry vectorDepositorInsertfriend leukemia integration 1a (fli1 Zebrafish)
UseGateway middle entryTagsExpressionMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40-hCas9 L3-L2
Plasmid#62133PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloningDepositorInserthuman codon optimized Cas9
UseCRISPR; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
p221- purVSG117UTR
Plasmid#59732PurposeInserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.DepositorInsertVSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
UseFor homologous recombination into vsg221 expressi…TagsExpressionMutationPromoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-FLAG
Plasmid#105783PurposeExpression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-FLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-TAZ CA
Plasmid#79504PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDSDepositorInsertWWTR1 (WWTR1 Human)
UseMultisite gateway entry cloneTags3xFLAGExpressionMutationS89APromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-JUN
Plasmid#79512PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDSDepositorInsertJUN (JUN Human)
UseMultisite gateway entry cloneTagsHA TagExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA
Plasmid#121799PurposepMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + CMV::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KN801: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-VPR
Plasmid#121829PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/VPR (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB
Plasmid#121830PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/KRAB (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO001: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-p300core
Plasmid#121832PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/p300core (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only