We narrowed to 14,583 results for: pET

1. pET28a-HIS-Xyl-Xmod-DocIII

   Plasmid

   #60865

   Purpose

   Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

   Depositor

   Hermann Gaub

   Article

   Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

   Inserts

   Xyl
   Xmod-DocIII
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Threonine 120 to Cysteine in Xylanase domain

   Promoter

   T7

   Available since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET22b-PelB-6H-Ag43-160N-sfGFP

   Plasmid

   #172886

   Purpose

   Display sfGFP protein on E. coli cell surface

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   SfGFP
   Ag43, partial (flu )
   

   Use

   Tags

   HHHHHH and TEV recognition site (ENLYFQG)

   Expression

   Bacterial

   Mutation

   Deleted 1-160 (N terminal)

   Promoter

   T7 Promoter

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET51b-His-TEV-CLIP-tag

   Plasmid

   #167274

   Purpose

   Overexpression of CLIP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   CLIP-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETDuet-1 Hepatitis C Virus NS3/NS4A

   Plasmid

   #61696

   Purpose

   inducible dual expression of NS3 protease along with its cofactor NS4A

   Depositor

   Michael Johnson

   Article

   Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

   Inserts

   NS4A
   NS3
   

   Use

   Tags

   his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-16b-10His-TEV-MCES_VACCA_D1_D12

   Plasmid

   #203671

   Purpose

   The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

   Depositor

   Stephen Cusack

   Article

   Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

   Insert

   MCES_VACCA_D1_D12
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21a(+)-HIV RT-6xHis-RBS-prot

   Plasmid

   #159149

   Purpose

   expresses His-tagged HIV reverse transcriptase and untagged HIV protease

   Depositor

   Andrea Pauli

   Article

   Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

   Inserts

   Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
   Human immunodeficiency virus 1 (HIV-1) protease
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   HIV-1 group M/subtype B – BH10 strain

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pET 28 full-length human PARP1

   Plasmid

   #192635

   Purpose

   Expresses human PARP1 with an N-terminal His-tag

   Depositor

   John Pascal

   Article

   Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

   Insert

   Human PARP1 (PARP1 Human)
   

   Use

   Tags

   histidine-tag

   Expression

   Bacterial

   Mutation

   Polymorphism A762

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-21a(+)-BMTU UDG-6xHis

   Plasmid

   #172197

   Purpose

   expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions

   Depositor

   Andrea Pauli

   Insert

   BMTU UDG
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pet28a-His6-Halo-TEV-Nrf2

    Plasmid

    #62455

    Purpose

    Halo-His6 tagged Nrf2 for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Nrf2 (NFE2L2 Human)
    

    Use

    Tags

    His6-Halo-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge