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Kir2.1 K+ channel scFv [N112/16]

Plasmid

#206769

Purpose

Mammalian Expression of Kir2.1 K+ channel scFV. Derived from hybridoma N112/16 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir2.1 K+ channel (Mus musculus) recombinant scFV (Kcnj2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Kir2.1 K+ channel scFv [N112B/29]

Plasmid

#206770

Purpose

Mammalian Expression of Kir2.1 K+ channel scFV. Derived from hybridoma N112B/29 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir2.1 K+ channel (Mus musculus) recombinant scFV (Kcnj2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-GLI1-P2A-mCherry

Plasmid

#203858

Purpose

Tet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

GLI1 (GLI1 Human)

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-OCRL/INPP5b [N166A/26]

Plasmid

#190301

Purpose

Mammalian Expression Plasmid of anti-OCRL/INPP5b (Human). Derived from hybridoma N166A/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-OCRL/INPP5b (Homo sapiens) recombinant Mouse monoclonal antibody (OCRL Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G596S

Plasmid

#116159

Purpose

Lentiviral expression of BRAF G596S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

G596S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.4 Na+ channel [N255/38]

Plasmid

#190306

Purpose

Mammalian Expression Plasmid of anti-Nav1.4 Na+ channel (Rat). Derived from hybridoma N255/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.4 Na+ channel (Rattus norvegicus) recombinant Mouse monoclonal antibody (Scn4a Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-W531C

Plasmid

#116210

Purpose

Lentiviral expression of BRAF W531C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

W531C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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8047_GFP-Kras2B_ires_puro

Plasmid

#64371

Purpose

This a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

none

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF6_nostop

Plasmid

#149323

Purpose

Gateway-compatible Entry vector, with insert of ORF6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF6 (ORF6 SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP.mIKKβ

Plasmid

#74412

Purpose

Expresses mouse eGFP-IKKβ in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

mouse IKKβ (Ikbkb Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Lgi1 [N283/7R]

Plasmid

#177527

Purpose

Mammalian Expression Plasmid of anti-Lgi1 (Mouse). Derived from hybridoma N283/7R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Lgi1 (Mus musculus) recombinant mouse monoclonal antibody (Lgi1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP2_nostop

Plasmid

#149305

Purpose

Gateway-compatible Entry vector, with insert of NSP2 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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C-Fos scFv [N486/32]

Plasmid

#182090

Purpose

Mammalian Expression of C-Fos scFv. Derived from hybridoma N486/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting C-Fos (Homo sapiens) (FOS Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-MAFB-P2A-mCherry

Plasmid

#203866

Purpose

Tet-inducible lentiviral plasmid expressing MAFB-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

MAFB (MAFB Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-RAC1-P34R

Plasmid

#116651

Purpose

Lentiviral expression of RAC1 P34R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAC1 (RAC1 Human)

Use

Lentiviral

Mutation

P34R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF7B_nostop

Plasmid

#149325

Purpose

Gateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-CDK4-R24L

Plasmid

#116217

Purpose

Lentiviral expression of CDK4 R24L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Mutation

R24L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-D594A

Plasmid

#116135

Purpose

Lentiviral expression of BRAF D594A

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

D594A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RAF1

Plasmid

#116785

Purpose

Lentiviral expression of RAF1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-GFAP [N206A/8R-2b]

Plasmid

#199410

Purpose

Mammalian expression plasmid of Anti-GFAP [N206A/8R-2b] (Human). Derived from hybridoma N206A/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFAP (Human) recombinant mouse monoclonal antibody (GFAP Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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