We narrowed to 10,337 results for: plasmids 101

1. pAAV-VTKS1

   Plasmid

   #170852

   Purpose

   AAV backbone for transgene expression in neurons under the control of the hSyn1 promoter.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-human-c-MYC-ER-mouse IRES-mcherry

   Plasmid

   #73570

   Purpose

   Mammalian retroviral expression of Myc-ER

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   cMYC (MYC Human)
   

   Use

   Retroviral

   Tags

   IRES-mCherry and Mouse ER

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-MCS-shCHD5 #1

   Plasmid

   #68876

   Purpose

   CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLV-tetO-Tfap2a

   Plasmid

   #70274

   Purpose

   Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2a

   Depositor

   Hubert Schorle

   Article

   Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

   Insert

   Tfap2a (Tfap2a Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-MCS-shCHD5 #2

   Plasmid

   #68877

   Purpose

   CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

   Plasmid

   #155306

   Purpose

   Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Rfx Cas13d CRISPR RNA BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME Actin-Vhh sfGFP (JDW 1215)

   Plasmid

   #224495

   Purpose

   Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. miniCGBE1-NG (pBM1182)

   Plasmid

   #140257

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)_NG-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNKX2-5-T2A-GFP-PGK-Puro

   Plasmid

   #203607

   Purpose

   Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

   Depositor

   Matthew Birket

   Article

   Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

   Insert

   NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-MBP-BrCas12b

    Plasmid

    #182276

    Purpose

    Expresses recombinant BrCas12b

    Depositor

    Piyush Jain

    Article

    Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, T7, Thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. rag2:MYRFL_CE_pISceI

    Plasmid

    #223045

    Purpose

    Express MYRFL gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    MYRFL
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV CMV mCherry-DDX21-V5

    Plasmid

    #175163

    Purpose

    Lentiviral expression of human DDX21-V5

    Depositor

    Larry Gerace

    Article

    Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

    Insert

    DDX21 (DDX21 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3

    Plasmid

    #170177

    Purpose

    Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2s-ST-P2A-NLS-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-Syn-TRPV1-P2A-DsRed

    Plasmid

    #200830

    Purpose

    Transduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporter

    Depositor

    Hong Chen

    Article

    Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

    Insert

    TRPV1-P2A-DsRed (Trpv1 Rat)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE

    Plasmid

    #183536

    Purpose

    Optogenetics

    Depositor

    Karl Deisseroth

    Article

    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

    Insert

    ChRmine-oScarlet-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CaMKIIa

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-SYN_mCherry-KIX-mCherry

    Plasmid

    #137007

    Purpose

    AAV backbone, mCherry-KIX acceptor for G-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    KIX domain (Crebbp Rat)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. (SUMO)5-(SIM)10

    Plasmid

    #126923

    Purpose

    Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    internal BamHI site between (SUMO)5 and (SIM)10

    Promoter

    tac

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PPIE_pLX307

    Plasmid

    #98361

    Purpose

    Lentiviral expression of PPIE

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    PPIE (PPIE Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. gh69

    Plasmid

    #106740

    Purpose

    expression of gRNA targeting B3GNT3

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B3GNT3 (B3GNT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PPP6C_pLX307

    Plasmid

    #98360

    Purpose

    Lentiviral expression of PPP6C

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    PPP6C (PPP6C Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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