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asPink chromoprotein promoter-less

Plasmid

#118005

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, asPink

Use

Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pS24·Pm-GFP

Plasmid

#122594

Purpose

Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

Pm promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pm

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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px330-CIB1-dCas9

Plasmid

#63665

Purpose

Mammalian gRNA expression vector also expressing CIB1-dCas9

Depositor

Dong Kong

Article

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 and CMV

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-RpS12

Plasmid

#71504

Purpose

flySTARR-seq screening vector with RpS12 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-K20

Plasmid

#14743

Depositor

M Bishr Omary

Article

Zhou et al Mol Biol Cell. 2003 Jul . 14(7):2959-71.

Insert

Keratin 20 (KRT20 Human)

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
V1579 pLP ProHis HTb - Acceptor

Plasmid

#11718

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

His

Expression

Bacterial

Available Since

June 2, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V181 pLPS 3' Triple-Flag - Acceptor

Plasmid

#11708

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available Since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pPath-Violacein-pT7native

Plasmid

#200002

Purpose

Violacein pathway. pT7-native CDS sequence

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp8a

Plasmid

#163592

Purpose

Expresses BMP-8A in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp8a (Bmp8a Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Magenta

Plasmid

#160446

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Magenta

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Yellow

Plasmid

#160443

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Yellow

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta12

Plasmid

#106116

Purpose

Lentiviral expression of PRKRA delta 12

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Mutation

Truncation

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDZ536 pURA ADE3p PP7-PS-2x-yeGFP

Plasmid

#72234

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

ADE3p

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78307

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADflluw

Available Since

July 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-CHES1L

Plasmid

#51836

Purpose

encodes the long isoform of human CHES1 and a Flag tag

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pET21a-hAIM2-His6

Plasmid

#51532

Purpose

Express His6-tagged hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

His6 and T7

Expression

Bacterial

Promoter

lac operator

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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CTM-PC1

Plasmid

#41573

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

FLM (4192-4303)

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pET28b-Rv2416c_wild

Plasmid

#37649

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

Insert

Rv2416c (eis Mycobacterium tuberculosis)

Tags

His

Expression

Bacterial

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pDUP 4-5

Plasmid

#23023

Depositor

Douglas Black

Article

Modafferi et al Mol Cell Biol. 1997 Nov . 17(11):6537-45

Insert

c-src (Src Mouse)

Expression

Mammalian

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-6

Plasmid

#18024

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-6 (Wnt6 Mouse)

Use

Retroviral

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only