We narrowed to 22,574 results for: tes

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pRS315-SEC22-NCYC110

Plasmid

#226273

Purpose

Plasmid expressing the SEC22 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-L1374

Plasmid

#226271

Purpose

Plasmid expressing the SEC22 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-UWOPS872421

Plasmid

#226272

Purpose

Plasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-S288C

Plasmid

#226270

Purpose

Plasmid expressing the SEC22 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAM227

Plasmid

#226650

Purpose

MSS-His6-UFD1L

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

UFD1 (UFD1 Human)

Tags

His-thrombin

Expression

Bacterial

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pML107-PMR1

Plasmid

#226264

Purpose

Plasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

PMR1 gRNA (PMR1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SSD1-NCYC110

Plasmid

#226279

Purpose

Plasmid expressing the SSD1 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SSD1 (SSD1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_LmFPPS

Plasmid

#186322

Purpose

pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

Insert

FPPS

Tags

His Tag

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-tractin

Plasmid

#187649

Purpose

Expresses F-tractin (from ITPKA)-mNeongreen under control of human synapsin promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

F-tractin

Use

AAV

Tags

mNeonGreen

Expression

Mammalian

Promoter

Synapsin

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-RLuc0---MS2

Plasmid

#210572

Purpose

Expresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal Renilla luciferase

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-RLuc100---MS2

Plasmid

#210573

Purpose

Expresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal Renilla luciferase

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-p37-SHPmut-FLAG

Plasmid

#225502

Purpose

Inducible expression of FLAG-tagged mutated p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

FLAG

Expression

Mammalian

Mutation

F215A/E218A/QKL220-222AAA

Promoter

TRE promoter, Tet ON

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-p37-WT-FLAG

Plasmid

#225501

Purpose

Inducible expression of FLAG-tagged wildtype p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

TRE promoter, Tet ON

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-UGCG-EGFP

Plasmid

#225878

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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p8221 pLIX-PTPN14

Plasmid

#221643

Purpose

Doxycycline-inducible overexpression of human WT PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 (PTPN14 Human)

Use

Lentiviral

Tags

HA

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MLG-EGFP-LST2

Plasmid

#220785

Purpose

Expresses N-terminal EGFP-LST2 in mammalian cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MLG-HMF-LST2

Plasmid

#220786

Purpose

Expresses N-terminal His10-Myc-FLAG-LST2 in mammalian cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Tags

10xHis-Myc-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-SFFV_NKX2-1_Short_V5-GFP

Plasmid

#221500

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

SFFV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelCTerm_1-223AA

Plasmid

#221476

Purpose

Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

Deletion of 224-371AA C-terminal domain

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelNTerm_160-371AA

Plasmid

#221475

Purpose

Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

Deletion of 1-159AA N-terminal domain

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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