We narrowed to 14,384 results for: nts

1. p3015b

   Plasmid

   #209317

   Purpose

   Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

   Depositor

   Thomas Hooven

   Article

   Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

   Insert

   Group B Streptococcus-compatible single guide RNA
   

   Expression

   Bacterial

   Promoter

   Xyl/tet promoter that is constitutively active

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HsGRK6-mVenus

   Plasmid

   #137780

   Purpose

   Visualization of GRK6

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Human GRK6 (GRK6 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CABE-T3.1

   Plasmid

   #193267

   Purpose

   Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

   Depositor

   Nicole Gaudelli

   Article

   Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   CABE-T3.1
   

   Expression

   Mammalian

   Mutation

   D10A (Cas9), TadA mutations within

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE-BR1

   Plasmid

   #160333

   Purpose

   This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

   Depositor

   Sang Taek Jung

   Article

   Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

   Insert

   BR1
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Renilla luciferase-Pol III

   Plasmid

   #37380

   Depositor

   Norbert Perrimon

   Article

   Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

   Insert

   PolIII-Renilla control reporter (RpIII128 Fly)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   RNA PolIII 128 subunit (RpIII128)

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

6. YFP-S100A10

   Plasmid

   #107200

   Purpose

   Expresses YFP-human S100A10 fusion protein for live cell imaging

   Depositor

   Volker Gerke, Ursula Rescher

   Article

   Zobiack et al J Cell Sci. 2002 Jan 1;115(Pt 1):91-8.

   Insert

   S100A10 (S100A10 Human)
   

   Tags

   Yellow Fluorescent Protein

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT2-4xUAS:P2A-mCerulean3

   Plasmid

   #127592

   Purpose

   This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

   Depositor

   Longhou Fang

   Article

   Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

   Type

   Empty backbone

   Use

   Zebrafish expression

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

   Plasmid

   #160611

   Purpose

   Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   P35s:LbCas12a:T35s
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28C-mCherry-DDX4LCD

   Plasmid

   #204408

   Purpose

   E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   DDX4(1-236) (DDX4 Human)
   

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-GRAB_OTmut

    Plasmid

    #185388

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Has Service

    AAV1 and AAV9

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458_mCherry-SRRM2-gRNA

    Plasmid

    #238251

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to SRRM2 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    SRRM2
    

    Use

    CRISPR

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19_msfGFP-SRRM2-repair-template

    Plasmid

    #238247

    Purpose

    For knock-in msfGFP to SRRM2 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    SRRM2
    

    Use

    CRISPR

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCPH2A.X-GS3

    Plasmid

    #204745

    Purpose

    Expression of Cas9 and human H2AX

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX (H2AX Human)
    

    Expression

    Mammalian

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-HA-C12orf66

    Plasmid

    #87048

    Purpose

    mammalian expression of C12orf66

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    C12orf66 (C12orf66 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAT9651-BEAR-GFP

    Plasmid

    #162989

    Purpose

    BEAR target plasmid with split EGFP and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28C-mCherry-53BP1LCD

    Plasmid

    #204407

    Purpose

    E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    53BP1(1208-1977) (TP53BP1 Human)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-p38KTRmCerulean3

    Plasmid

    #59149

    Purpose

    ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
    

    Available Since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. KHBD00532

    Plasmid

    #39630

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG3629 (Dll Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Aug. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pRK5-HA-KPTN

    Plasmid

    #87042

    Purpose

    mammalian expression of KPTN

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    KPTN (KPTN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ265E9

    Plasmid

    #213472

    Purpose

    Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing

    Depositor

    Yiping Qi

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Insert

    TadDE-32aa linker-zSpCas9-D10A-2xUGI
    

    Use

    CRISPR; Gateway compatible tadde-32aa linker-zspc…

    Tags

    NO

    Expression

    Plant

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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