We narrowed to 18,079 results for: comp

1. pTO149_RFP_CauNEO

   Plasmid

   #177277

   Purpose

   Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

   Depositor

   Teresa O'Meara

   Article

   Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

   Insert

   RFP, CauNEO
   

   Use

   Tags

   ENO1-RFP

   Expression

   Bacterial

   Mutation

   All CUG Leucine codons were replaced with the syn…

   Promoter

   AgTEF2

   Available since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-PB2-B/BRISBANE/60/2008

   Plasmid

   #187917

   Purpose

   Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 2
   

   Use

   Tags

   UNTAGGED

   Expression

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGoldenGreenGate-M (pGGG-M)

   Plasmid

   #165422

   Purpose

   A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).

   Depositor

   Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Level 2 golden gate (moclo) du…

   Tags

   Expression

   Mutation

   Promoter

   Contain GG cassette and MCS with LacZ for blue/wh…

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTP2620

   Plasmid

   #113311

   Purpose

   human Rad50 in baculovirus transfer vector

   Depositor

   Tanya Paull

   Article

   Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

   Insert

   Rad50 (RAD50 Human)
   

   Use

   Tags

   HA, 6xhis

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

   Plasmid

   #199359

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOEu-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMIA22

   Plasmid

   #214448

   Purpose

   Expresses BxbI-BP-NLS

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   BxbI
   

   Use

   Tags

   BP-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFast-hHus1

   Plasmid

   #222469

   Purpose

   Expresses hHus1 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hHus1 (HUS1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBWB514

   Plasmid

   #209326

   Purpose

   p15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.

   Depositor

   Adam Arkin

   Article

   Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

   Insert

   mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tet

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEL2319

   Plasmid

   #195697

   Purpose

   Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   Foldon-MlaC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-Foldon-(Gly-Ser-Linker)-MlaC

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. rs214-minor

    Plasmid

    #113380

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2149943 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO GFP HSPA1A

    Plasmid

    #19483

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA1A (HSPA1A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. gWiz_BM40-vGAT-VL-hIgG1-CL

    Plasmid

    #218437

    Purpose

    mammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domain

    Depositor

    Axel Brunger

    Article

    Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

    Insert

    vGAT-VL-hIgG1-CL
    

    Use

    Affinity Reagent/ Antibody

    Tags

    BM40

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV human IRS-1 CAAX

    Plasmid

    #11238

    Depositor

    Ronald Kahn

    Article

    Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    C-terminal prenylation motif makes IRS-1 localize…

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pC003 - LshC2C2 locus into pACYC184 for spacer cloning

    Plasmid

    #79152

    Purpose

    LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    LshC2c2 locus
    

    Use

    Tags

    Expression

    Mutation

    *

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-MH6-EsCas13d

    Plasmid

    #108303

    Purpose

    Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    EsCas13d
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSD057C

    Plasmid

    #218136

    Purpose

    For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved ZF degron SD36.

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD36
    

    Use

    Tags

    rpoZ

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-eNme2-C.NR

    Plasmid

    #185672

    Purpose

    Expresses nuclease active eNme2-C.NR in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    eNme2-C.NR nuclease
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP VAMP4

    Plasmid

    #42313

    Depositor

    Thierry Galli

    Article

    Mallard et al J Cell Biol. 2002 Feb 18;156(4):653-64.

    Insert

    VAMP 4 (VAMP4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF.DEST51-mScarlet

    Plasmid

    #207960

    Purpose

    Vector for overexpression of the mScarlet fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-(SUMO)3

    Plasmid

    #127093

    Purpose

    Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    EGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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