We narrowed to 14,023 results for: egf
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Plasmid#122956PurposeLentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expressionDepositorInsertNOTCH2NL-delta C terminus (NOTCH2NLB Human)
UseLentiviralTags6xhis tagExpressionMutationdeletion of C terminus from full length NOTCH2NLBPromoterCAG promoterAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα-Smad7-SOCS1
Plasmid#242412PurposeSelf-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitors, srIκBα, Smad7, and SOCS1.DepositorInsertsmScarlet3
IRES-E3-NSs-L*-EGFP
IRES-srIκBα-Smad7-SOCS1
UseMouse Targeting; Self-amplifying rnaTagsExpressionMammalianMutationPromoterAvailable SinceSept. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori
Plasmid#234737PurposeAll-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.DepositorAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP
Plasmid#196990PurposeDerived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNADepositorInsertVEGFR2
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM075 SFFV-mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP (FLP-IN)
Plasmid#233439PurposeSFFV driven LIDAR reporter with 2 tandem substrate stem loopsDepositorInsertmCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)
Plasmid#223766PurposeExpression of recombinant protein for purificationDepositorInsertBNIP3 soluble part (BNIP3 Human)
UseTagsEGFP-TEV-GSTExpressionInsectMutationW18A/L21A; deletion of LIRPromoterAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9
Plasmid#231910PurposeFor packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)DepositorInsertEGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA
Plasmid#228787PurposeEcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppressionDepositorInsertsEcTyrRS
EcYtR
EGFP***
TEV protease
UseBaculoviralTagsExpressionMammalianMutationTAG suppressorPromoterAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)
Plasmid#216871PurposeThis plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23DepositorInsertCas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
UseCRISPRTagsExpressionMutationmdx mutation in mouse dystrophinPromoterAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)
Plasmid#218636PurposeProximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseLentiviralTagsAPEX2-3xFLAGExpressionMammalianMutationH781E, Q782R, F785APromoterUBCAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)
Plasmid#218635PurposeProximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseLentiviralTagsAPEX2-3xFLAGExpressionMammalianMutationN1008R, R1009E, E1010R, R1013EPromoterUBCAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A_S
Plasmid#147515PurposeInsect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961ADepositorInsertCeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A (gw Human, Nematode)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A_R
Plasmid#147389PurposeInsect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961ADepositorInsertCeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A (gw Fly, Nematode)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
EK0494 SFFV-mCherry-SGc(CFL1.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191181PurposeExpression of CFL1 sensor in mammalian cellsDepositorInsertmCherry:CFL1.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)
Plasmid#191166PurposeMammalian expression of a EGFP mutant N213D derived from EK0379.DepositorInsertEGFP(N213D)
UseTagsExpressionMammalianMutationc.637A>G(N213D; CCA>CCG)PromoterCMV-TOAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)
Plasmid#185489PurposeCMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFPDepositorInserthuman codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-eGFPExpressionMammalianMutationH840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…PromoterCMV and T7Available SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)
Plasmid#185492PurposeCMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFPDepositorInserthuman codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-eGFPExpressionMammalianMutationD10APromoterCMV and T7Available SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R
Plasmid#147394PurposeInsect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389ADepositorInsertCeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A (TNRC6C Human, Nematode)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)
Plasmid#176139PurposeEGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassetteDepositorUseLentiviralTagsEGFP and MYCExpressionMammalianMutationPromoterEF1AAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide4
Plasmid#125443PurposeCRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only