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Showing: 10711 - 10740 of 77447 results

  1. psiCHECK-2 GATD1 Alu_Mut2

    Plasmid
    #205471
    Purpose
    Luciferase reporter for Alu Domain Activity
    Depositor
    Glen Borchert
    Insert
    Mutant 2 GATD1 Alu Domain
    Use
    Luciferase and RNAi
    Mutation
    Mutated the arms of the alu domain by adding ACAC…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    205471-plasmid-map-sequence-id-405408

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  2. pAG CMV C-HA Puro

    Plasmid
    #237880
    Purpose
    Empty mammalian expression plasmid for cloning C-terminally HA-tagged proteins
    Depositor
    Meghan Rego, Addgene Research Program
    Type
    Empty backbone
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237880-plasmid-map-sequence-id-469913

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  4. Ec holA(Y316A)-FLAG-pET(11a)(ACH-)

    Plasmid
    #238346
    Purpose
    over-expresses C-terminal FLAG tagged E. coli delta (Y316A) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    C-terminal FLAG tagged E. coli delta (Y316A) (holA E. coli)
    Tags
    3X FLAG
    Expression
    Bacterial
    Mutation
    Y316A
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238346-plasmid-map-sequence-id-472706

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  5. pML107-KAE1

    Plasmid
    #232891
    Purpose
    Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    KAE1 gRNA (KAE1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232891-plasmid-map-sequence-id-470392

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  6. pAG Lenti CMV EGFP-HA Puro

    Plasmid
    #236082
    Purpose
    Lentiviral backbone expressing C-terminally HA tagged EGFP, control for empty backbone 236080
    Depositor
    Melina Fan
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    HA
    Promoter
    CMV
    Available Since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236082-plasmid-map-sequence-id-467819

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  7. pAAV-Ef1a-Con/Fon-Synaptophysin-10xMyc-WPRE

    Plasmid
    #237446
    Purpose
    Intersectional expression of Myc-tagged synaptophysin in the presence of Cre and Flp
    Depositor
    Silvia Arber
    Insert
    Con/Fon-Synaptophysin-10xMyc
    Use
    AAV
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    237446-plasmid-map-sequence-id-470110

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  8. Ec holB-pET(11a)(ACH-)

    Plasmid
    #237234
    Purpose
    over-express E. coli holB (Pol III delta prime subunit)
    Depositor
    Michael O'Donnell
    Insert
    holB (delta prime) (holB E. coli)
    Expression
    Bacterial
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237234-plasmid-map-sequence-id-469255

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  10. TRE-FRT-MCS-WPRE-SV40 - GCH27

    Plasmid
    #230828
    Purpose
    AAV cloning vector for Flpo-dependent expression under the TRE promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector, flp/frt
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230828-plasmid-map-sequence-id-466645

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  11. pML107-ARB1

    Plasmid
    #232888
    Purpose
    Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    ARB1 gRNA (ARB1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232888-plasmid-map-sequence-id-464740

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  12. pGL3_THADA_pos1toneg0

    Plasmid
    #232388
    Purpose
    THADA enhancer, one sensitizing element made into buffering Coordinator
    Depositor
    Joanna Wysocka
    Insert
    THADA (THADA Human)
    Use
    Luciferase
    Mutation
    THADA enhancer, one sensitizing element made into…
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232388-plasmid-map-sequence-id-462859

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  13. pGL3_MSR1_bothneg0scramble

    Plasmid
    #232383
    Purpose
    MSR1 enhancer, both buffering Coordinators scrambled
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Mutation
    MSR1 enhancer, both buffering Coordinators scramb…
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232383-plasmid-map-sequence-id-462848

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  14. pML107-RER2

    Plasmid
    #232884
    Purpose
    Plasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    RER2 gRNA (RER2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232884-plasmid-map-sequence-id-462289

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  15. pML104-LEU2-3

    Plasmid
    #232894
    Purpose
    Plasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    LEU2 gRNA (RER2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232894-plasmid-map-sequence-id-462276

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  16. pML107-AVO1

    Plasmid
    #232889
    Purpose
    Plasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    AVO1 gRNA (AVO1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232889-plasmid-map-sequence-id-462277

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  17. pML107-YFR054C

    Plasmid
    #232900
    Purpose
    Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    YFR054C gRNA (YFR054C Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232900-plasmid-map-sequence-id-462143

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  18. pML107-SRP14

    Plasmid
    #232896
    Purpose
    Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    SRP14 gRNA (SRP14 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232896-plasmid-map-sequence-id-462141

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  19. pML107-TLG1

    Plasmid
    #232897
    Purpose
    Plasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    TLG1 gRNA (TLG1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232897-plasmid-map-sequence-id-462140

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  20. pML107-VHT1

    Plasmid
    #232898
    Purpose
    Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    VHT1 gRNA (VHT1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232898-plasmid-map-sequence-id-462142

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  21. pML104-LEU2-2

    Plasmid
    #232883
    Purpose
    Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    LEU2 gRNA (LEU2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232883-plasmid-map-sequence-id-462088

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  22. pML107-INO80

    Plasmid
    #232890
    Purpose
    Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    INO80 gRNA (INO80 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232890-plasmid-map-sequence-id-462082

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  23. AAV-hSyn-PiGM-Iq(535/81, eGFP)

    Plasmid
    #221612
    Purpose
    AAV encoding PiGM-Iq with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).
    Depositor
    John Lin
    Insert
    PiGM-Iq (535/81,eGFP)
    Use
    AAV
    Mutation
    RGS2 1-53 truncation
    Promoter
    Human Synapsin
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    221612-plasmid-map-sequence-id-461237

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  24. pGL3_THADA_alltoneg0

    Plasmid
    #232372
    Purpose
    THADA enhancer, all sensitizing elements made into buffering Coordinator
    Depositor
    Joanna Wysocka
    Insert
    THADA (THADA Human)
    Use
    Luciferase
    Mutation
    THADA enhancer, all sensitizing elements made int…
    Available Since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232372-plasmid-map-sequence-id-461186

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  25. pPMS-2049

    Plasmid
    #232291
    Purpose
    Protein Expression
    Depositor
    Jeremy Nathans
    Insert
    Reck (Reck Mouse)
    Expression
    Mammalian
    Available Since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232291-plasmid-map-sequence-id-461168

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  26. pPMS-2016

    Plasmid
    #232290
    Purpose
    Protein Expression
    Depositor
    Jeremy Nathans
    Insert
    Apcdd1 (Apcdd1 Mouse)
    Tags
    8X His-Avi-1D4
    Expression
    Mammalian
    Available Since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232290-plasmid-map-sequence-id-461150

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  27. MSCV-IRES-tagBFP

    Plasmid
    #219965
    Purpose
    express TagBFP in mammalian cells
    Depositor
    Mohamed Fareh
    Insert
    TagBFP
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    219965-plasmid-map-sequence-id-442485

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  28. pT7-35aaNSP1noMSA11-NSP5(io)

    Plasmid
    #220827
    Purpose
    Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that lacks all methionines fused with the full-length recoded NSP5 protein
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 35aaNSP1noM-NSP5(io)
    Use
    Other
    Mutation
    the 35aa NSP1 lacks all Methionines
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220827-plasmid-map-sequence-id-449169

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  29. pT7-35aaNSP1SA11-P2A-NSP5(io)-GS-smURFP

    Plasmid
    #220828
    Purpose
    Express separately the short Rotavirus SA11 NSP1 protein (35 amino acids) and the full-length recoded NSP5 protein fused with GS-linker and smURFP
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 35aaNSP1-P2A-NSP5(io)-GS-smURFP
    Use
    Other
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220828-plasmid-map-sequence-id-444017

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  30. pT7-35aaNSP1noMSA11-NSP5(wt)

    Plasmid
    #220826
    Purpose
    Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that lacks all methionines fused with the full-length SA11 NSP5 protein
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 35aaNSP1noM-NSP5(wt)
    Use
    Other
    Mutation
    the 35aa NSP1 lacks all Methionines
    Available Since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220826-plasmid-map-sequence-id-449171

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Showing: 10711 - 10740 of 77447 results