We narrowed to 14,384 results for: nts

1. pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

   Plasmid

   #139485

   Purpose

   PCR template for Halo-Tag gene knock-in donor vector

   Depositor

   Kazuhiro Aoki

   Article

   Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

   Insert

   HaloTag-loxP-P2A-dTKneo-pA-loxP
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

   Plasmid

   #136382

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV-T2A-MCP-VPR
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D570A

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-myc-HLA-B8

   Plasmid

   #135512

   Purpose

   Mammalian expression of myc-tagged HLA-B*08:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-B*08:01 (HLA-B Human)
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SPLINTR GFP v1 backbone

   Plasmid

   #179777

   Purpose

   SPLINTR backbone vector

   Depositor

   Mark Dawson

   Article

   Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIND-hygro-HA-H3.3

   Plasmid

   #47981

   Purpose

   Ponasterone A-inducible expression of human HA tagged H3F3A

   Depositor

   Bing Zhu

   Article

   Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

   Insert

   H3F3A (H3-3A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   288 bp T to C synonymous mutation

   Promoter

   Minimal Heat Shock Promoter

   Available Since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-EFS-DIO-GRAB_OTmut

   Plasmid

   #185389

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin control sensor GRAB_OTmut
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDIRECT_26H

   Plasmid

   #91150

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. cpGFP-Adhiron_flex

   Plasmid

   #165044

   Purpose

   Flexible linker control for POLArISact expression in mammalian cells

   Depositor

   Sumio Terada

   Article

   Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

   Insert

   cpGFP-Adhiron (connected with a flexible linker)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_B2312

   Plasmid

   #91070

   Purpose

   Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
   

   Use

   CRISPR

   Promoter

   PvUbi1

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. DCT.VV.Orf45

    Plasmid

    #12733

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf45
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

11. DCT.VV.Orf170

    Plasmid

    #12858

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf170
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pCEP4-myc-H2-Dd

    Plasmid

    #135518

    Purpose

    Mammalian expression of myc-tagged H2-Dd

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Dd (H2-D1 Mouse)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    C121S

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET30b jGCaMP8f F366H

    Plasmid

    #204139

    Purpose

    Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Tags

    N-terminal His tag

    Expression

    Bacterial

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    T7

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ3 SHP2 CS

    Plasmid

    #8319

    Depositor

    Ben Neel

    Article

    Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

    Insert

    SHP2 (PTPN11 Human)
    

    Expression

    Mammalian

    Mutation

    C459S, mutation of essential catalytic cysteine

    Available Since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. RnBarr1-mVenus

    Plasmid

    #137790

    Purpose

    Visualization of beta-arrestin1

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin 1 (Arrb1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMOD_B2301

    Plasmid

    #91067

    Purpose

    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Promoter

    35S

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330.Pten.a

    Plasmid

    #66587

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Expression

    Mammalian

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. CoV2-N-G204R

    Plasmid

    #177953

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (G204R) (N )
    

    Expression

    Mammalian

    Mutation

    G204R

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBSFI-myr-akt-K179M

    Plasmid

    #49188

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from K to M at position 179 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Mutation

    K179M

    Promoter

    T7

    Available Since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. α1Ic-HE2-pcDNA3

    Plasmid

    #45808

    Depositor

    Edward Perez-Reyes

    Article

    Murbartian et al FEBS Lett. 2002 Sep 25;528(1-3):272-8.

    Insert

    α1Ic (Cacna1i Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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