Addgene

We narrowed to 6,032 results for: WUS;

Showing: 1051 - 1080 of 6032 results

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217_pAAV-ProD25-CatCh-GFP-WPRE

Plasmid

#126001

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProD25

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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155_pAAV-ProA4-CatCh-GFP-WPRE

Plasmid

#125888

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA4

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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120_pAAV-ProC26-CatCh-GFP-WPRE

Plasmid

#125961

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC26

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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152_pAAV-ProC30-CatCh-GFP-WPRE

Plasmid

#125965

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC30

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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168_pAAV-ProC35-CatCh-GFP-WPRE

Plasmid

#125969

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC35

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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171_pAAV-ProC36-CatCh-GFP-WPRE

Plasmid

#125970

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC36

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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172_pAAV-ProC37-CatCh-GFP-WPRE

Plasmid

#125971

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC37

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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173_pAAV-ProC38-CatCh-GFP-WPRE

Plasmid

#125972

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC38

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
174_pAAV-ProC39-CatCh-GFP-WPRE

Plasmid

#125973

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC39

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
42_pAAV-ProC41-CatCh-GFP-WPRE

Plasmid

#125975

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC41

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
157_pAAV-ProD2-CatCh-GFP-WPRE

Plasmid

#125978

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProD2

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pET29b-rhMOG

Plasmid

#225484

Purpose

Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

Depositor

John Pak

Article

Wu et al J Immunol Methods. 2024 Oct 13:113764. d

Insert

Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

deleted amino acids 1-29 (signal peptide) and 150…

Promoter

T7

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-ER-LAR-GECO1

Plasmid

#61244

Purpose

Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

Insert

LAR-GECO1

Use

Tags

ER-retention sequence: KDEL and ER-targeting sequ…

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: V51W/I113V/N35…

Promoter

CMV

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-HsNRF2 (NFE2L2)

Plasmid

#194303

Purpose

Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

NFE2L2 (NFE2L2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pdCas12a(RuvC)

Plasmid

#186447

Purpose

Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

dCas12a

Use

CRISPR

Tags

Expression

Mutation

D917A, E1006A

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pdCas12b(RuvC)

Plasmid

#186450

Purpose

Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

dCas12b

Use

CRISPR

Tags

Expression

Mutation

E848A, D977A

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mCherry-PA-Rac1

Plasmid

#22035

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mCherry-PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

Tet-CMV

Available since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

Plasmid

#214102

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PE-Plus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-eGFP-PGK-mCherry

Plasmid

#191862

Purpose

Heat inducible eGFP reporter with constitutive mCherry marker

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

eGFP
mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

HSPA7 promoter and PGK promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-mito-LAR-GECO1.2

Plasmid

#61245

Purpose

Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

Insert

LAR-GECO1.2

Use

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1.2: N45I/A47R/E1…

Promoter

CMV

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-ORAI1-EC-HA

Plasmid

#174335

Purpose

encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Use

Tags

GFP in the N-terminus and HA tag in the extracell…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-L858R-T790M-dual-epegRNA

Plasmid

#214100

Purpose

Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R epegRNA/EGFR T790M epegRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1-C450A

Plasmid

#22028

Purpose

Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))

Use

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV-FOXP3-IRES-Thy1.1

Plasmid

#17443

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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p23-puro-EGFP-FBL

Plasmid

#179235

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

FBL (FBL Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-mito-CAR-GECO1

Plasmid

#46022

Purpose

Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

CAR-GECO1

Use

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

Promoter

CMV

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only
pHR-Hsp-Fluc-PGK-Rluc-mCherry

Plasmid

#191863

Purpose

Heat inducible Fluc reporter with constitutive Rluc

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

Firefly luciferase Fluc
Renilla luciferase Rluc

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Mutation

Promoter

HSPA7 promoter and PGK promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1-T17N

Plasmid

#22029

Purpose

Expression of photoactivatable Rac1 (dominant-negative mutant).

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))

Use

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_GLP1R-NTEV-TCS-GV-2xHA

Plasmid

#194378

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GLP1R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-REX-GECO1

Plasmid

#61246

Purpose

Expresses REX-GECO1 in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

Insert

REX-GECO1

Use

Tags

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: P60R, V61W, R6…

Promoter

CMV

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge