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pMXLK_pFa

Plasmid

#114229

Purpose

POC12367, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046277

Plasmid

#145428

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDP-let-7_LPB

Plasmid

#26560

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPB

Use

Transcription vector

Mutation

loop mutant B

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-clbB

Plasmid

#114155

Purpose

Expresses full-length ClbB

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbB

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-let-7-A114-N40

Plasmid

#50563

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-let7-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pTH730-staRLuc

Plasmid

#38226

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pSDH51

Plasmid

#139045

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag (no STOP codon, PATC introns)

Expression

Worm

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSM04

Plasmid

#139046

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (no STOP codon)

Expression

Worm

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSM05

Plasmid

#139050

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (includes STOP codon)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046467

Plasmid

#149039

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046229

Plasmid

#145569

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046489

Plasmid

#145554

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMXLP_pFa

Plasmid

#114239

Purpose

POC12379, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH

Plasmid

#114156

Purpose

Expresses full-length ClbH

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pQuantFLAG-bsr-kana

Plasmid

#45593

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-FLAG tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pQuantFLAG-puro-kana

Plasmid

#45592

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-FLAG tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pQuantFLAG-hygro-kana

Plasmid

#45591

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-FLAG tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pQuantEGFP-bsr-kana

Plasmid

#45590

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-EGFP tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pQuantA-puro-kana

Plasmid

#45586

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-A tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pQuantEGFP-puro-kana

Plasmid

#45589

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-EGFP tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pQuantA-bsr-kana

Plasmid

#45587

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-A tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL0956

Plasmid

#252420

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (H4C6 Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL0943

Plasmid

#252414

Purpose

Auto-Inhibited TVMV protease for the RAPID-release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

TVMV-AI

Tags

iFP2.0-FKBP12

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL0941

Plasmid

#252413

Purpose

Auto-Inhibited TVMV protease for the RAPID-release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

TVMV-AI

Tags

TagBFP-FKBP12

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL0919

Plasmid

#252412

Purpose

Auto-Inhibited TVMV protease for the RAPID-release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

TVMV-AI

Tags

mCherry-FKBP12

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL0639

Plasmid

#252410

Purpose

NASP tagged on the N-terminus with mCherry.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

sNASP (NASP Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL1011

Plasmid

#252432

Purpose

RbAp46 tagged on the N-terminus with mCherry and a Nuclear Export Sequence to make it cytoplasmic.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

RbAp46 (RBBP7 Human)

Tags

mCherry-NES

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pAL10

Plasmid

#236006

Purpose

Backbone for assembling gRNA arrays with LacZ gene for blue white screening

Depositor

Carl de Boer

Article

Salaudeen et al Synth Biol (Oxf). 2025 Dec 18;11(1):ysaf

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1553

Plasmid

#238470

Purpose

pMOBC360_VL: Vector Left (VL) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1554

Plasmid

#238471

Purpose

pMOBC360_VR: Vector Right (VR) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1525

Plasmid

#226496

Purpose

pMOBC360_VM: Vector Middle (VM) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1520

Plasmid

#221581

Purpose

pMOBK360_VR: Vector Right (VR) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1518

Plasmid

#221579

Purpose

pMOBK360_VL: Vector Left (VL) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1519

Plasmid

#221580

Purpose

pMOBK360_VM: Vector Middle (VM) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPOW

Plasmid

#205699

Purpose

shRNAmir backbone for RNA interference, with mOrange reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MAPVW

Plasmid

#205698

Purpose

shRNAmir backbone for RNA interference, with Venus reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Retroviral

Promoter

MSCV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LHAPAW

Plasmid

#205679

Purpose

shRNAmir backbone for RNA interference, with Ametrine reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

EH

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LAAPCW

Plasmid

#205691

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

AH

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LHAPVW

Plasmid

#205680

Purpose

shRNAmir backbone for RNA interference, with Venus reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

EH

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LHAPTW

Plasmid

#205677

Purpose

shRNAmir backbone for RNA interference, with mTagBFP2 reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

EH

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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