We narrowed to 7,368 results for: ALP

1. IKKalpha (S176E,S180E)-pcw107-V5

   Plasmid

   #64608

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   CHUK (CHUK Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   S176E, S180E

   Promoter

   PGK

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe puro IkB alpha M

   Plasmid

   #12332

   Depositor

   Inder Verma

   Insert

   IkB alpha M (Nfkbia Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Dominant-negative mutant. Many signal transductio…

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5/FRT/TO-GAlphai2-RLuc8

   Plasmid

   #140974

   Purpose

   Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphai2-Rluc8 (GNAI2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTrcHisA-PPARalpha(aa101-468)

   Plasmid

   #13574

   Depositor

   Martin Scanlon

   Insert

   human peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468) (PPARA Human)
   

   Use

   Tags

   x6His N-terminal, enterokinase cleavage site

   Expression

   Bacterial

   Mutation

   deleted residues 1-100.

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pscALPS gag-gfp/deltavpx

   Plasmid

   #115807

   Purpose

   SFFV promoter expresses gag-gfp fusion with no ORF after CypA promoter

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   gag-gfp
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-hSyn-Galpha-i3 ONE-GO

   Plasmid

   #204334

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-i3 ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pscALPS gag-gfp/vpx

   Plasmid

   #115808

   Purpose

   SFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   gag-gfp
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1-OSF-TRIM5alpha(human)

   Plasmid

   #79034

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(human) (TRIM5 Human)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(-) rat C/EBP alpha

   Plasmid

   #12550

   Depositor

   Peter Johnson

   Article

   Miller et al J Biol Chem. 2003 Apr 25;278(17):15178-8

   Insert

   CCAAT/Enhancer-binding Protein alpha (Cebpa Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLENTI EF1alpha FLAG/HA-PALI1

    Plasmid

    #114445

    Purpose

    Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    PALI1 (LCOR Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO-GAlpha12-RLuc8

    Plasmid

    #140985

    Purpose

    Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha12-RLuc8 (GNA12 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RHPMP125 (mEOS3.1-alpha Tubulin)

    Plasmid

    #88834

    Purpose

    Localization by Super-Resolution microscopy of alpha-Tubulin fused to mEOS3.1

    Depositor

    Ricardo Henriques

    Insert

    alpha-tubulin (TUBA1B) (TUBA1B Human)
    

    Use

    Tags

    mEOS3.1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. RHPMP126 (mOrange2-alpha Tubulin)

    Plasmid

    #88835

    Purpose

    Localization by Super-Resolution microscopy of alpha-Tubulin fused to mOrange2

    Depositor

    Ricardo Henriques

    Insert

    alpha-tubulin (TUBA1B) (TUBA1B Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAX APPs-695-alpha

    Plasmid

    #30147

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein - soluble (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 1–612

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pDEST-GST-FBW7 alpha

    Plasmid

    #197456

    Purpose

    The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 (FBXW7 Human)
    

    Use

    In vitro translation

    Tags

    GST

    Expression

    Bacterial

    Mutation

    See depositor comments below

    Promoter

    T7

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pscALPS-HIV-1 Group O-vpr

    Plasmid

    #115836

    Purpose

    Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HIV1 Group O Vpr (L20571)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. CoV2-Spike-D614G-Alpha

    Plasmid

    #177961

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)

    Depositor

    Melanie Ott

    Article

    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

    Insert

    SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    del69, 70, del144, D614G, N501Y, A570D, P681H, T7…

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJG/ Inducible ALPHA MHC

    Plasmid

    #55792

    Purpose

    transgene expression driven by inducible ALPHA MHC promoter

    Depositor

    Jeffrey Robbins

    Article

    Sanbe et al Circ Res. 2003 Apr 4;92(6):609-16. Epub

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Inducible ALPHA MHC

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pGV13 (CK2alpha K68M, CK2beta)

    Plasmid

    #27094

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Inserts

    CK2beta (CSNK2B Human)
    CK2alpha K68M (CSNK2A1 Human)
    

    Use

    Tetracycline-regulated expression

    Tags

    HA on CK2alpha and Myc on CK2beta

    Expression

    Mammalian

    Mutation

    K68M on CK2alpha

    Promoter

    bidirectional tet-responsive promoter and bidirec…

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

    Plasmid

    #140979

    Purpose

    Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaGustducin-RLuc8 (GNAT3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge