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pEHISSSO1986

Plasmid

#97057

Purpose

Expressing Sulfolobus solfataricus orf SSO1986 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO1986

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPApqsL

Plasmid

#97020

Purpose

Expressing Pseudomonas aeruginosa pqsL protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative FAD-dependent monooxygenase

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSAPPFK

Plasmid

#97003

Purpose

Expressing MRSA PPFK protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative phosphofructokinase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSO1404

Plasmid

#97056

Purpose

Expressing Sulfolobus solfataricus orf SSO1404 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO1404

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF239

Plasmid

#97025

Purpose

Expressing Pyrobaculum spherical virus orf239 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 239

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF165A

Plasmid

#97024

Purpose

Expressing Pyrobaculum spherical virus orf165a protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 165a

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF137

Plasmid

#97023

Purpose

Expressing Pyrobaculum spherical virus orf137 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 137

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA1376

Plasmid

#97002

Purpose

Expressing MRSA sar1376 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative 4-oxalocrotonate tautomerase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF131

Plasmid

#97022

Purpose

Expressing Pyrobaculum spherical virus orf131 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 131

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA4511

Plasmid

#97015

Purpose

Expressing Pseudomonas aeruginosa orf PA4511 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P. aeruginosa orf PA4511

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSATAG

Plasmid

#97004

Purpose

Expressing MRSA TAG protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

DNA-3-methyladenine glycosylase I

Tags

N-ter TEV protease cleavable 6His

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a-TALEN_HD (SPC right)

Plasmid

#126695

Purpose

right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC right

Use

TALEN

Tags

NLS, truncated N term and TAL, FokI

Expression

Mammalian

Mutation

R451P in SPC

Promoter

EF1

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a-TALEN_NN (SPC left)

Plasmid

#126694

Purpose

left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC left

Use

TALEN

Tags

0.5 repeat NN, TAL, FokI and NLS, truncated N ter…

Expression

Mammalian

Promoter

EF1

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON HaloTag-Jph3 WPRE

Plasmid

#236238

Purpose

AAV expression of human Junctophilin3 N-terminally tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

Junctophilin 3 (JPH3 Human)

Use

AAV

Tags

HaloTag

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mCherry-Jph3 WPRE

Plasmid

#236237

Purpose

AAV expression of human Junctophilin3 N-terminally tagged with mCherry

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

Junctophilin 3 (JPH3 Human)

Use

AAV

Tags

mCherry

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1aL-Cre-IRES-NeoR-W

Plasmid

#126700

Purpose

lentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassette

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

Cre-IRES

Use

Cre/Lox and Lentiviral

Tags

SV40 NLS

Promoter

Ef1a

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET30(a)-AtNUDX23

Plasmid

#175021

Purpose

Purification of AtNUDX23 enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

AtNUDX23 (NUDX23 Mustard Weed)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Cavin4-mCherry

Plasmid

#176007

Purpose

Mammalian expression of Mouse Cavin4-mCherry. Parton lab clone JSG

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4 (Cavin4 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pHL-EF1a-SphcCas9(D10A)-iP-A

Plasmid

#60600

Purpose

Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Inserts

CRISPR Cas9 D10A
puromycin resistance gene

Use

CRISPR

Expression

Mammalian

Mutation

Changed Asp 10 to Ala, Codon usage optimized for …

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA0482

Plasmid

#96968

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 0482

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

MRSA ORF0482

Tags

N-ter TEV protease cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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