We narrowed to 29,069 results for: PLE;

1. pLenti6-CENP-A-mCherry

   Plasmid

   #89767

   Purpose

   Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

   Depositor

   Torsten Wittmann

   Article

   Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

   Insert

   CENP-A (CENPA Human)
   

   Use

   Lentiviral

   Tags

   3xHA and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_dCas9-KRAB-MeCP2_Blast

   Plasmid

   #221501

   Purpose

   Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   dCas9-KRAB-MeCP2-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti puro HA-Ubiquitin

   Plasmid

   #74218

   Purpose

   Lentiviral expression of HA tagged Ubiquitin

   Depositor

   Melina Fan

   Insert

   ubiquitin (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Single ubiquitin coding sequence (full length UBB…

   Promoter

   CMV

   Available since

   March 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. plenti6-SLC7A11/xCT-V5

   Plasmid

   #170427

   Purpose

   Eepresses SLC7A11 (also named xCT) in mammalian cells

   Depositor

   Boyi Gan

   Article

   Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

   Insert

   solute carrier family 7 member 11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCMVNeoDEST(705-1)-hAXL

   Plasmid

   #124796

   Purpose

   Expresses AXL in mammalian cells

   Depositor

   Kenneth Pienta

   Article

   Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

   Insert

   AXL (AXL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mApple-LAMP1-phLuorin-N-8

   Plasmid

   #54918

   Purpose

   Localization: Lysosome Membrane, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   LAMP1 phLuorin
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Lysosomes-20 double labeled with phLuorin

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRUPATH Triple Gaq

   Plasmid

   #196058

   Purpose

   Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaQ-RLuc8 (GNAQ Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6/V5-DEST-HSF1

   Plasmid

   #31210

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   HSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

9. TRUPATH Triple GaZ

   Plasmid

   #196053

   Purpose

   Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaz-RLuc8 (GNAZ Human)
   GGamma1-GFP2 (GNGT1 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-Lifeact-tdTomato

    Plasmid

    #64048

    Purpose

    Fluorescent reporter for F-actin labeling in living cells

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-pHluorin_M153R-CD63

    Plasmid

    #172117

    Purpose

    Fluorescent reporter for CD63-positive exosome secretion

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    pHluorin_M153R in a small extracelluar loop of CD…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRUPATH Triple Ga12

    Plasmid

    #196061

    Purpose

    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRUPATH Triple Ga13

    Plasmid

    #196062

    Purpose

    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-PE2max-BSD

    Plasmid

    #191102

    Purpose

    Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    PEmax-P2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed R221K, N394K, H840A from SpCas9

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. plex_307-ccdB-blasticidin

    Plasmid

    #201136

    Purpose

    pLEX_307 plasmid with blasticidin marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6/V5-p53_wt p53

    Plasmid

    #22945

    Purpose

    3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tag

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. plex_306-ccdB-hygro

    Plasmid

    #201135

    Purpose

    pLEX_306 plasmid with hygro marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. plex_306-ccdB-g418

    Plasmid

    #201133

    Purpose

    pLEX_306 plasmid with g418 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. plex_306-ccdB-zeo

    Plasmid

    #201134

    Purpose

    pLEX_306 plasmid with zeo marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX307-TMPRSS2-puro

    Plasmid

    #158457

    Purpose

    Constitutive lentiviral expression of human TMPRSS2.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge