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Showing: 701 - 720 of 771 results

  1. pLY132

    Plasmid
    #130960
    Purpose
    Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB3
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Anderson promoter J23100
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    130960-plasmid-map-sequence-id-257712

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  2. pLY130

    Plasmid
    #130952
    Purpose
    Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Anderson promoter J23100
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    130952-plasmid-map-sequence-id-257710

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  3. pX330-BbsI-PITCh

    Plasmid
    #127875
    Purpose
    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly
    Depositor
    Peter Kaiser
    Article
    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127875-plasmid-map-sequence-id-418291

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  9. pNM3746

    Plasmid
    #154109
    Purpose
    loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector
    Depositor
    Michael Nonet
    Article
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Inserts
    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::His-58::FRT3
    mex-5p::FLP D5::sl2::mNG::glh2 3'
    Use
    Cre/Lox; Rmce
    Tags
    Expression
    Worm
    Mutation
    Promoter
    not applicable
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154109-plasmid-map-sequence-id-306068

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  12. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  13. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  16. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

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  17. pShoHELIX-sgRNA_entry (BO3)

    Plasmid
    #181784
    Purpose
    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181784-plasmid-map-sequence-id-358224

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  19. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid
    #181781
    Purpose
    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181781-plasmid-map-sequence-id-354678

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  20. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid
    #181792
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181792-plasmid-map-sequence-id-358226

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Showing: 701 - 720 of 771 results