We narrowed to 114,331 results for: MPI

1. pCAGGs-Venus

   Plasmid

   #127346

   Purpose

   Expresses Venus constitutively via the CAGGS promoter in mammalian cells

   Depositor

   Phil Sharp

   Insert

   Venus Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

   Plasmid

   #28289

   Purpose

   3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)

   Depositor

   Alex Chenchik, Gus Frangou

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBPZpGAL4DBDUw

   Plasmid

   #26233

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Aug 9. ():.

   Insert

   Zip-GAL4DBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAG-jGCaMP8m-WPRE

   Plasmid

   #179255

   Purpose

   AAV-mediated expression of GCaMP8m under the CAG promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p405GAL1

   Plasmid

   #15963

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   p405GAL1 (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GAL1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK7-HA-S6K1-WT

   Plasmid

   #8984

   Depositor

   John Blenis

   Article

   Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE80L Hu IL-15-His tag

   Plasmid

   #183256

   Purpose

   Bacterial expression of human IL-15 with N-terminal His tag

   Depositor

   Alexander McLellan

   Insert

   IL-15 (IL15 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQCXIP-VEGFA121

   Plasmid

   #73017

   Purpose

   Retroviral expression of human VEGFA121

   Depositor

   Michael Grusch

   Insert

   VEGFA (VEGFA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_CMV_CapChR2-GFP

   Plasmid

   #224357

   Purpose

   Expresses CapChR2-GFP in mammalian cells

   Depositor

   Adam Cohen

   Article

   Hayward et al Cell Calcium. 2024 Dec 5;125:102983. doi

   Insert

   CapChR2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS L30 mRuby3-Rab18

    Plasmid

    #166431

    Purpose

    Rab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.

    Depositor

    Raphael Gaudin

    Insert

    Rab18
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    L30 weak promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-4T-1-3xHA

    Plasmid

    #129567

    Purpose

    Empty backbone for HA tag

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-miRFP670

    Plasmid

    #91854

    Purpose

    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

    Depositor

    Ralf Kuehn

    Inserts

    hSpCas9-2A-miRFP670
    BB-guide RNA
    

    Use

    CRISPR

    Tags

    2A-miRFP670 and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh and U6

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. ORFtag_TetR-SD_Frame2

    Plasmid

    #220982

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. ORFtag_TetR-SD_Frame3

    Plasmid

    #220983

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB700-mouse-TTN-Cerulean

    Plasmid

    #128325

    Purpose

    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

    Depositor

    Alejandro Chavez

    Insert

    gRNA against mouse TTN for activation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. THINGL-RR pcDNA3.1

    Plasmid

    #230047

    Purpose

    Expressing the human version of mScarletI-T2A-TINGL biosensor for glucose (named THINGL-RR), ratiometric variant with mScarletI.

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    THINGL-RR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV-tMCK-mem-TurboID

    Plasmid

    #149410

    Purpose

    Muscle-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Insert

    mem-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    tMCK

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET23b-NTF2

    Plasmid

    #108918

    Purpose

    Expresses NTF2

    Depositor

    Larry Gerace

    Article

    Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

    Insert

    NTF2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiCROP-PuroR-P2A-GFP-U6

    Plasmid

    #200029

    Purpose

    Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

    Depositor

    Jay Shendure

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    PuroR-P2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-MBP-NLS-Geo_st

    Plasmid

    #87703

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Use

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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