We narrowed to 18,489 results for: Met

1. pACYCDuet_WTDHFR_WTTS

   Plasmid

   #91226

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL1P1_crU626

   Plasmid

   #191776

   Purpose

   Cas12a guide expression in dicots

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHpgRNA13

   Plasmid

   #160127

   Purpose

   To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1

   Depositor

   Yongjin Zhou

   Article

   Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

   Insert

   PTEF1-HH-gRNA-ADE2- HDV-TAMO
   

   Expression

   Yeast

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. YEp181-CUP1-His-Smt3

   Plasmid

   #99536

   Purpose

   Yeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 marker

   Depositor

   Helle Ulrich

   Article

   Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

   Insert

   SMT3 (SMT3 Budding Yeast)
   

   Tags

   7xHis

   Expression

   Yeast

   Promoter

   CUP1

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJCC_083 Cas12i1

   Plasmid

   #171670

   Purpose

   For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   Cas12i1
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SCP2-CMVenh-luc

   Plasmid

   #22230

   Depositor

   James Kadonaga

   Article

   Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

   Insert

   SCP2 (super core promoter 2)-CMVenh luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. Launchpad (AVA2590)

   Plasmid

   #85442

   Purpose

   Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   FRT-flanked EF1alfa-GFP PGK-Puromycin cassette
   

   Use

   Lentiviral

   Available Since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pAdEasy mCherry-Tubulin

   Plasmid

   #26767

   Depositor

   Torsten Wittmann

   Article

   Matov et al Nat Methods. 2010 Sep . 7(9):761-8.

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Adenoviral

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   April 8, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pDB4282

   Plasmid

   #98701

   Purpose

   A plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   gRNA PCR template
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

    Plasmid

    #55221

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    StrepII and msfGFP

    Expression

    Insect

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB8786

    Plasmid

    #126920

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Expression

    Yeast

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUPD2+pEstrInd

    Plasmid

    #170880

    Purpose

    Phytobrick - Estradiol Inducible Promoter

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    pEstradiol_Inducible
    

    Expression

    Plant

    Mutation

    c.837C>T and c.1353G>A to remove BsaI and E…

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNIR_PKAbiosensor

    Plasmid

    #127439

    Purpose

    Mammalian expression of the NIR biosensor for detecting PKA activity

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    NIR PKA biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. MXS_E2-Crimson

    Plasmid

    #62410

    Purpose

    MXS_chaining vector with E2-Crimson

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    E2-Crimson
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-4T-3-SpSuc1

    Plasmid

    #162558

    Purpose

    Expresses GST-tagged S.pombe Suc1 in E.coli

    Depositor

    Iain Hagan

    Article

    Singh et al Methods Mol Biol. 2021;2329:123-142. doi

    Insert

    Schizosaccharomyces pombe Suc1 (suc1 Fission Yeast)
    

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNIR_JNKbiosensor

    Plasmid

    #127440

    Purpose

    Mammalian expression of the NIR biosensor for detecting JNK activity

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    NIR JNK biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSF11-wemiRFP670-T2A-HO1

    Plasmid

    #197243

    Purpose

    Expresses the protein of wemiRFP670-T2A-HO1 in neurons of C elegan

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wemiRFP670-T2A-HO1
    

    Expression

    Worm

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNL(CMV)EGFP/CMV/WPRE∆U3

    Plasmid

    #41790

    Purpose

    Lentiviral vector for CMV promoter driven expression of EGFP

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    EGFP
    

    Use

    Lentiviral

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSP2

    Plasmid

    #26282

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP2
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of dimer of deletion mutant (1-43)…

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pTK81_GFP-4.1R

    Plasmid

    #46352

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1R (EPB41 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    pBABE

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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