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pCAGEN-FastFUCCI (JDW 1494)

Plasmid

#242592

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAP-Rab6a_T27N

Plasmid

#248794

Purpose

Expresses SNAP-Rab6a-T27N (dominant negative) in mammalian cells. Generated from Addgene #46782 plasmid.

Depositor

Michael Robichaux

Article

Hekmatara et al bioRxiv [Preprint]. 2025 Sep 25:2025.09.

Insert

Rab6a (RAB6A Human)

Tags

SNAPf

Expression

Mammalian

Mutation

Change T 27 to N

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAP-Rab6a

Plasmid

#248793

Purpose

Expresses SNAP-Rab6a (WT) in mammalian cells. Generated from Addgene #46781 plasmid.

Depositor

Michael Robichaux

Article

Hekmatara et al bioRxiv [Preprint]. 2025 Sep 25:2025.09.

Insert

Rab6a (RAB6A Human)

Tags

SNAPf

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1α-H2B-PA-mCherry-PGKneo

Plasmid

#247340

Purpose

Expresses photoactivatable H2B-Cherry under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2CR-QUASM1:GCaMP7s-NLS-P2A-mKate2-NLS

Plasmid

#241915

Purpose

Zebrafish transgenesis plasmid encoding GCaMP7s-NLS and mKate2-NES separated by the P2A peptide, driven by the QUASM1 enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker

Depositor

Balázs Enyedi

Article

Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

Insert

QUASM1 enhancer driving GCaMP7s-NLS and mKate2-NLS

Use

Zebrafish tol2 transgenesis

Promoter

QUASM1

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)

Plasmid

#242578

Purpose

PiggyBac transposon flanked, Tet-on, gateway destination vector.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DV-IRES-myr-mKate (JDW 494)

Plasmid

#242575

Purpose

Gateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-mKate to label cell membranes.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-Luciferase-WPRE-bGH-pA (JDW 1509)

Plasmid

#242572

Purpose

Gateway 3' entry clone containing an IRES Luciferase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-Luciferase-WPRE-bGH-pA

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-LiCre (JDW 1119)

Plasmid

#242560

Purpose

Gateway middle entry clone containing a light inducible Cre Recombinase (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre (AsLOV2-CreE340AD341A)

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-miR-MCS (JDW 1231)

Plasmid

#242562

Purpose

Gateway middle entry clone containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with artifial intron and MCS

Use

Gateway subcloning

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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