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  1. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    .... What aspect of GeoCas9 are you most excited about? Thermostability? Longevity in human plasma? Something...active at high temperatures Figure 1: Thermostability of SpCas9 vs GeoCas9. SpCas9 or GeoCas9 RNPs...cocaine esterase engineered to have increased thermostability also had a longer lifetime in human blood. ...lower than optimal temperatures. GeoCas9’s thermostability could also be used with in vitro molecular ...CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9 with increased lifetime in human plasma... thermostable cas9...

  2. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...post. There’s so many great tips for getting the most from your conference experience so we unfortunately...them all. What are your top tips for getting the most out of your scientific conference? Resources on...

  3. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...reproducibility. Read on for some tips for getting the most out of your lentiviral transduction experiments....point to consider is how the virus is being titered. Most titering approaches transduce into highly permissive...

  5. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    .... Our most popular kit of the year was the Golden Gate TALEN Kit, which continues to be our most requested...distributed through Addgene over our first 10 years. 10 most distributed plasmid technologies pSV-PGC1 - Ok,...Fire's set of vectors for C. elegans research was our most requested item of 2005. shRNA - In 2006, we saw ...two plasmids from Didier Trono's lab are our two most popular plasmids of the past 10 years. Induced Pluripotent... Yamanaka's lab and James Thomson's lab were the most frequently requested. Ubiquitin - One particular...interest in from 2008 to 2009 was ubiquitin. The most requested ubiquitin plasmids were pRK5-HA-Ubiquitin-WT...

  7. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ... the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year it was wonderful to learn that...Ignavibacteriae phylum genome. IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to ...optimal sgRNA spacer length of 22-25 nts, and is thermostable between 31-100°C. They published their finding... in a bioRxiv article and in PNAS. Two other thermostable Cas9 enzymes have previously been characterized...ThermoCas9, IgnaviCas9 is a type II-C Cas9. IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans...What can you do with IgnaviCas9? Why would a thermostable Cas9 be useful? It can enable applications where...steps in the protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly useful. Additional...

  9. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...recommend it except in the most dire of circumstances.) Multiplex assays Mo’ proteins, mo’ problems may not be...region, where an antibody binds to its target. While most secondary antibodies recognize broad isotype classes...across applications. Western blots, for instance, almost always use the indirect approach, while flow cytometry...assay and protein of interest is often one of the most challenging components of any immunoimaging assay...incorrect. With the exception of flow cytometry, most immunoimaging assays do not allow for absolute quantitative... and occasionally ask themselves and others that most perplexing of questions: “who moved my goat anti-rabbit...

  10. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...created and designed for the Hall of Human Life at the MOS. (If you want a fun refresher on the Central Dogma...scientists, part of the Addgene family Perhaps the most heartfelt portion of the entire night was when Melina...

  11. CRISPR Guide

    Type
    Collection
    ...Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H., Zhou, R., Lang, B., Meng, B., Weng, H., & Hu...inversions with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to target...simplicity and adaptability, CRISPR rapidly became the most popular genome engineering approach. Figure 1: Overview...ease of generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR...mutations (see: Validate genetic modification ). In most cases, NHEJ gives rise to small indels in the target...homology elsewhere in the genome. Realistically, most gRNA targeting sequences will have additional sites...specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular Cas endonuclease, with many modified versions...

  12. Antibody Guide

    Type
    Collection
    ...immune system’s response to foreign bodies, are the most commonly used affinity reagents. Antibodies are ...against the antigen, packaged, and sold. This is the most cost-efficient way to produce antibodies, with the... IgA2 IgD Coexpressed with IgM on the surface of most immature B cells; function is unknown Monomer with...anaphylactic shock Monomer with a valency of 2 IgG The most abundant isotype in blood, also found in tissues...Produced during the primary immune response; found mostly in blood Pentamer with a valency of 10 Antigen ... for exact storage requirements. As a guideline, most antibodies can be stored at 4 °C for short-term ...allow detection of the antibody:protein complex. The most commonly used types of signaling molecules are horseradish...

  13. Worm Expression Resources

    Type
    Collection
    ...CRISPR/Cas9 in Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab... related miniMos plasmids can be found on the Jorgensen Lab page. More information on miniMos and MosSCI...

  14. Neurodegeneration Research Collection

    Type
    Collection
    ...dollars spent each year. Unfortunately, the causes of most neurodegenerative diseases are only partly understood...no effective therapies to prevent, treat, or cure most of these disorders. An integrated study of the overlapping...introduction to the genetics associated with some of the most common disorders followed by a list of some of the...ten million people worldwide are living with PD, mostly people over the age of 60. There is presently no...Alzheimer’s Disease Alzheimer’s disease (AD) is the most common cause of dementia. Symptoms include memory...

  15. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...repetitive and non-repetitive genomic loci, as well as chromosome painting in living cells. Visualizing a specific...labeled proteins to the given region. For example, chromosome-specific repetitive loci can be efficiently visualized...co-delivering multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs...gRNAs with target sites throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable...

  16. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...addresses", where dCas9 is going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression...bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be found in this article...Shearwin, K. E. (2013). One-Step cloning and chromosomal integration of DNA. ACS Synthetic Biology, 2(...dCas9 under a P-tet promoter integrated in the chromosome at phage attachment sites. The levels of the ...

  17. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... guides around the region to find the most efficient guide (most guides would work, but they sometimes...multiplexing CRISPR to target multiple genome loci, the most efficient and easiest way is to co-transfect several...homology arms be for efficient recombination? The most we've tried to insert so far has been 1kb. We used...

  18. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Michael J Fox Foundation MJFF 40386 pBACgus_Sumostar/LRRK2 1867-2186 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40387 pBACgus_Sumostar/LRRK2 1867-2186_G2019S LRRK2 His, TEV polH...Parkinson's Michael J Fox Foundation MJFF 40388 pBACgus_Sumostar/LRRK2 1867-2186_C2024S/C2025S LRRK2 His, TEV...Parkinson's Michael J Fox Foundation MJFF 40389 pBACgus_Sumostar/LRRK2 1867-2186_T2031E LRRK2 His, TEV polH...Parkinson's Michael J Fox Foundation MJFF 40390 pBACgus_Sumostar/LRRK2 1867-2141 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40391 pBACgus_Sumostar/LRRK2 1867-2161 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40392 pBACgus_Sumostar/LRRK2 1867-2176 LRRK2 His, TEV polH Parkinson's...

  19. Rett Syndrome

    Type
    Collection
    ...localization) the C - t erminal D omain (CTD) The most common missense mutations cluster in the MBD and...R168X, R255X, R270X, R294X, and R306C) account for almost 46% of all Rett syndrome cases. Research Milestones... and the direction and degree of skewing of X-chromosome inactivation. J Med Genet . 44, 148–152. (Link...

  20. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...presence of specific molecules or other factors. Most often, a conformational change in the sensing region... astrocyte calcium signaling in the hippocampal mossy fiber pathway. Neuron. 2014 Apr 16;82(2):413-29....from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):444...intracellular environment of S. cerevisiae . The MOSAIC Kit from the Cohen laboratory includes 35 plasmids...
Showing: 1 - 20 of 764 results