We narrowed to 7,729 results for: Pol
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Plasmid#227136PurposeBarcoded assay, MAPK sensor; barcode BC0132DepositorInsertEGR1 promoter driving barcode 0132 and a luciferase reporter gene
ExpressionMammalianAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1
Plasmid#228508PurposeBacterial Hybrid Driver PlasmidDepositorInsertT7 RNAP, LARP-I5CHO (#1)
UseSynthetic BiologyTagsUmu Degron, GTG-start codonMutationR378K; S430P, N433T, S633P, F849I, F880Y; W727G, …Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK_Gal4-DBD-ESR1
Plasmid#227099PurposeESR1 receptor overexpression for luciferase and barcode assaysDepositorInsertESR1 (ESR1 Human)
ExpressionMammalianAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B2-ELP-HIS-ybbR
Plasmid#169134PurposeE. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgß-FIVAR-ELP-HIS-ybbR
Plasmid#169136PurposeE. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertFIVAR (found in various architectures)
Tags3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…ExpressionBacterialAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B1-ELP-HIS-ybbR
Plasmid#169133PurposeE. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225062PurposeExpression and Nuclear-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
TagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM
Plasmid#225061PurposeExpression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
TagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN667
Plasmid#219963PurposeExpressed sfGFP fused human KIF1Bß(1-721) in insect cellsDepositorInsertcodon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
TagssfGFP::2xStrepII tagExpressionInsectMutationencodes 1-721PromoterpolyhedrinAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_DCAF8L2:1-631
Plasmid#211239PurposeMammalian expression of full-length human DCAF8L2. N-terminal HiBiT tag.DepositorInsertDCAF8L2:M1-S631 (DCAF8L2 Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutation145-147: missing in Poly E regionPromoterCMVAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hTspan12LEL(118-218)_pAcGP67a
Plasmid#216383PurposeBaculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBPDepositorPublicationInsertTetraspanin-12 (TSPAN12 Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3CExpressionInsectPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RNF169-Halo HRD
Plasmid#207083PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.DepositorInsertHaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
Plasmid#216378PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTagsHA signal sequence-FLAGExpressionInsectPromoterPolyhedrinAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFBOH-LIC_MAGEA3:104-314
Plasmid#210941PurposeInsect expression of human MAGEA3 fragment E104 to E314. Untagged.DepositorAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_SHKBP1:182-585
Plasmid#210935PurposeInsect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.DepositorInsertSHKBP1:T182-L585 (SHKBP1 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationQ507L natural variant (VAR_036714)PromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_TRAF7:382-670
Plasmid#210930PurposeInsect expression of human TRAF7 fragment P382 to C670. N-terminal 6X His tag with TEV cleavage.DepositorInsertTRAF7:P382-C670 (TRAF7 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_TLE3:472-772
Plasmid#210928PurposeInsect expression of human TLE3 fragment G472 to Y772. N-terminal 6X His tag with TEV cleavage.DepositorInsertTLE3:G472-Y772 (TLE3 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_PAFAH1B1:86-410
Plasmid#210923PurposeInsect expression of human PAFAH1B1 fragment G86 to R410. N-terminal 6X His tag with TEV cleavage.DepositorInsertPAFAH1B1:G86-R410 (PAFAH1B1 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_UTP4:1-686
Plasmid#210915PurposeInsect expression of full-length human UTP4. N-terminal 6X His tag with TEV cleavage.DepositorInsertUTP4:M1-R686 (CIRH1A Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_DCAF13:54-356
Plasmid#210908PurposeInsect expression of human DCAF13 fragment F54 to A356. N-terminal 6X His tag with TEV cleavage.DepositorInsertDCAF13:F54-A356 (DCAF13 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationA86S sequence conflictPromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits