We narrowed to 14,384 results for: nts

1. pDIRECT_21B

   Plasmid

   #91129

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFAB3689

   Plasmid

   #47834

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 3 and BCD1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   see insert

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T2 SHP2 E76A

   Plasmid

   #8324

   Depositor

   Ben Neel

   Article

   O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

   Insert

   SHP2 (PTPN11 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E76A (activated mutant)

   Available Since

   June 17, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pWZL Blast H-Ras G12V T35S

   Plasmid

   #12278

   Depositor

   Bob Weinberg

   Article

   Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

   Insert

   H-Ras G12V T35S (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Contains G12V activating mutation. The T35S mutat…

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pHAGE2-TetO-GFP-mSox2

   Plasmid

   #206374

   Purpose

   Expresses EGFP fused mouse SOX2 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CoV2-N-M234I

   Plasmid

   #177955

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (M234I) (N )
   

   Expression

   Mammalian

   Mutation

   M234I

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. KG371-GFP-mios

   Plasmid

   #87052

   Purpose

   mammalian expression of mios

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   mios (MIOS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay-GRAB_OTmut

   Plasmid

   #185385

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin control sensor GRAB_OTmut
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. BtGRK2-sYFP2

   Plasmid

   #137778

   Purpose

   Visualization of GRK2

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Bovine GRK2 (GRK2 Bovine)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEMHE-POLArISact

    Plasmid

    #164970

    Purpose

    T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. tdp43-EGFP construct10

    Plasmid

    #28203

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-105 only

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pCD442

    Plasmid

    #153024

    Purpose

    dCas9, MCP-SoxS(R93A/S101A)

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dCas9, MCP-SoxS(R93A/S101A)
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Available Since

    Aug. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_mGFP-FGFR3-mScarlet

    Plasmid

    #192509

    Purpose

    Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc-mScarlet
    

    Tags

    mGFP and mScarlet

    Expression

    Mammalian

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. DCT.VV.Orf42

    Plasmid

    #12730

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf42
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

15. 782 pMEActMEK

    Plasmid

    #31166

    Depositor

    Nathan Lawson

    Article

    Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

    Insert

    MEK (MAP2K1 Human)
    

    Use

    Entry vector

    Mutation

    Ser218Glu Ser222Asp; activated MEKK

    Available Since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-CAG-eYFP-3x-miR204-5p-TS

    Plasmid

    #117380

    Purpose

    An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. RnBarr1-mTurquoise2

    Plasmid

    #137789

    Purpose

    Visualization of beta-arrestin1

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin 1 (Arrb1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAT-9208

    Plasmid

    #124221

    Purpose

    Cloning plasmid for a self-targeting gRNA library

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSP72-RBM8A-3'UTR AluY-Sz WT 2598-4066

    Plasmid

    #175587

    Purpose

    in vitro transcription of wt RBM8A 3'UTR

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    RBM8A-3'UTR AluY-Sz WT
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mWasabi-Mito-7

    Plasmid

    #56508

    Purpose

    Localization: Mitochondria, Excitation: 493, Emission: 509

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Mito (COX8A Human)
    

    Tags

    mWasabi

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only