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pGEX-4T-1-Shp2 T7DA/S189D SH2s

Plasmid

#67577

Purpose

Expresses GST-tagged Shp2 T73D/S189D SH2 domains in bacteria

Depositor

Graeme Carnegie

Publication

Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

Insert

Src homology 2 domain-containing phosphatase 2

Tags

GST

Expression

Bacterial

Mutation

Deleted amino acid residues 219-597, changed T73 …

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
DCBL-bio-His

Plasmid

#51867

Purpose

Expresses full-length Discoidin, CUB and LCCL domain-containing protein 2 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

DCBL (DCBLD2 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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CD84-bio-His

Plasmid

#51649

Purpose

Expresses full-length SLAM family member 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

CD84 (CD84 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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G6B-bio-His

Plasmid

#51644

Purpose

Expresses full-length Protein G6b precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

G6B (MPIG6B Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-FRB-DMVF-181-N

Plasmid

#87610

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-DMVF181-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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HA-L-SA-2A-NeoR-CAG-M2rtTA-Cas9-TRE-HA-R

Plasmid

#258168

Purpose

DNA template for insertion of doxycycline inducible Cas9 and constitutively active M2rtTA expression cassettes into human AAVS1 site

Depositor

Danwei Huangfu

Publication

Zhang et al Dev Cell. 2026 May 19:S1534-5807(26)0015

Insert

SpCas9

Tags

Flag tagged

Expression

Mammalian

Promoter

TRE

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pAT3156-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank1_COLO320 DM_ AT_sg-276-282])

Plasmid

#251113

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Publication

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-GFP-MeCP2-R168X (pc4742 )

Plasmid

#249084

Purpose

Bacteria expression plasmid of GFP tagged MeCP2-R168X (aa1-aa167)

Depositor

Cristina Cardoso

Publication

Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Bacterial

Mutation

MeCP2-R168X (aa1-aa167)

Promoter

T7

Available since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-GFP-MeCP2-R255X (pc4743 )

Plasmid

#249085

Purpose

Bacteria expression plasmid of GFP tagged MeCP2-R255X (aa1-aa254)

Depositor

Cristina Cardoso

Publication

Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Bacterial

Mutation

MeCP2-R255X (aa1-aa254)

Promoter

T7

Available since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAT3158-{PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank3_COLO320 DM_ AT_sg-290-296])

Plasmid

#251115

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Publication

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_GammaSMA_ALFA_230231

Plasmid

#250442

Purpose

Expresses tagged gamma smooth muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T230/A231.

Depositor

Koen van den Dries

Publication

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Gamma Smooth Muscle Actin (ACTG2 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pAT3159-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank4_COLO320 DM_ AT_sg-297-303])

Plasmid

#251116

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Publication

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT3157-{PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank2_COLO320 DM_ AT_sg-283-289])

Plasmid

#251114

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Publication

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGPR124 ΔGAIN-His

Plasmid

#246693

Purpose

Expression vector for secreted human GPR124 ECD lacking the GAIN domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Publication

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD Δaa 427-755

Promoter

CMV

Available since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGPR124 ΔHRM-His

Plasmid

#246692

Purpose

Expression vector for secreted human GPR124 ECD lacking the HRM domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Publication

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD Δaa 349-426

Promoter

CMV

Available since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGPR124 ΔLRR-His

Plasmid

#246691

Purpose

Expression vector for secreted human GPR124 ECD lacking LRR domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Publication

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD Δaa 34-247

Promoter

CMV

Available since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-GFP-2A-FOXP2-WPRE-bGHpA

Plasmid

#231020

Purpose

For lentivirus-mediated co-expression of GFP and FOXP2

Depositor

Tomasz Nowakowski

Publication

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Inserts

Enhanced GFP
FOXP2 (FOXP2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-GFP-2A-DGCR8-WPRE-bGHpA

Plasmid

#231021

Purpose

For lentivirus-mediated co-expression of GFP and DGCR8

Depositor

Tomasz Nowakowski

Publication

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Inserts

Enhanced GFP
DGCR8 (DGCR8 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_hhN-HA

Plasmid

#240225

Purpose

Gateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

hedgehog N-terminal fragment (hh Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Mutation

aa 1-257

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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FHpHG_mTent5c_LxxAA (L3m)

Plasmid

#221441

Purpose

Transfection into the cells for the reporter or qPCR assay.

Depositor

Marcos Morgan

Publication

Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

Insert

Tent5c_LXXAA (L3m) (Fam46c Mouse)

Tags

FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

Expression

Mammalian

Mutation

L332A, LL335-336AA (CTC atc tct CTC CTG to CTC at…

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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