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YIp128-P30-His-POL30

Plasmid

#99542

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pPD057 VP64-ZF37

Plasmid

#138834

Purpose

Constitutive expression of VP64-ZF37 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF37

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Hras

Plasmid

#180573

Purpose

ddGFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Hras

Expression

Mammalian

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shGFP

Plasmid

#61230

Purpose

Lentivirus expressing shRNA to GFP

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

GFP

Use

Lentiviral

Promoter

H1/TO

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
MXS_BiTet

Plasmid

#62423

Purpose

MXS_chaining vector with BiTet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Bidirectional tetracycline inducible promoter

Use

Synthetic Biology

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

Plasmid

#173456

Purpose

Contains mScarletH-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-H-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pIM1708-surT-PsurP-agaN-pNW33N

Plasmid

#44009

Depositor

Ichiro Matsumura

Article

Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

Insert

surT-PsurP-agaN

Expression

Bacterial

Mutation

Deleted EcoRI site (synonymous change, C to T at …

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+HA-hSeparase

Plasmid

#33018

Depositor

Marc Kirschner

Article

Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

Insert

Separase (ESPL1 Human)

Tags

3xHA

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pDU1

Plasmid

#169817

Purpose

pSpCas9(BB)-2A-tCD19 (pDU1)

Depositor

Andrea Rossi

Article

Ramachandran et al Life Sci Alliance. 2021 Apr 26;4(6). pii

Insert

tCD19

Tags

fusion protein

Expression

Mammalian

Promoter

Cbh

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MAC-C-GFP

Plasmid

#172973

Purpose

MAC-tagged GFP expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GFP

Expression

Bacterial and Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBSII-SK-mTol2-MCS

Plasmid

#51817

Purpose

To generate transgenic axolotls using Tol2 or SceI mediated transgenics

Depositor

Elly Tanaka

Article

Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

Type

Empty backbone

Use

Unspecified

Available Since

April 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE Hygro ST6GalNaC5

Plasmid

#26981

Depositor

Joan Massague

Article

Bos et al Nature. 2009 Jun 18. 459(7249):1005-9.

Insert

ST6GalNaC5 (ST6GALNAC5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pMA4854

Plasmid

#184849

Purpose

Coexpression of PhiC31o recombinase and EGFP

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Inserts

EGFP
PhiC31o

Expression

Mammalian

Promoter

CMV and RSV

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

Plasmid

#55216

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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UbcH10 AU1

Plasmid

#8506

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Tags

AU1 (DTYRYI)

Expression

Mammalian

Available Since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pMito.iGABASnFR.mRuby3

Plasmid

#112179

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Tags

mRuby3

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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MAP72_MS2

Plasmid

#220623

Purpose

Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Use

Shuttle vector

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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mTB3-Myc tag

Plasmid

#210205

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

Myc-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUb6-Zpo1-V5/His

Plasmid

#29323

Depositor

Zena Werb

Article

Slorach et al Genes Dev. 2011 Mar 1;25(5):471-84. doi:

Insert

Zeppo1

Tags

His and V5

Expression

Mammalian

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only
pucKan_ARS_pgkZeo_ncx1_mCherry_2TA_Puro_ARS

Plasmid

#163755

Purpose

piggybac transposon encoding human Ncx1 gene promoter-driven mCherry and puromycin-acetyltransferase for labelling and selection of pluripotent stem cell-derived cardiomyocytes

Depositor

Kurt Pfannkuche

Article

Hamad et al Methods Mol Biol. 2021 Oct 19. doi: 10.1

Insert

mCherry

Use

Piccybac transposon

Tags

puromycin-N-acetyltransferase

Expression

Mammalian

Promoter

human NCX1

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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