We narrowed to 9,520 results for: try

1. pKK-TEV-FLAG

   Plasmid

   #105783

   Purpose

   Expression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-FLAG

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p221- purVSG117UTR

   Plasmid

   #59732

   Purpose

   Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

   Depositor

   Gloria Rudenko

   Article

   Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

   Insert

   VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
   

   Use

   For homologous recombination into vsg221 expressi…

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA

   Plasmid

   #121799

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + CMV::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TGmiR_Gb2-K

   Plasmid

   #25766

   Purpose

   Entry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Gb2 miR-shRNA (Gnb2 Mouse)
   

   Use

   Entry vector

   Tags

   GFP

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. D998-EGFR-ET1

   Plasmid

   #44186

   Depositor

   Timothy Springer

   Article

   Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

   Insert

   D998-EGFR-ET1 (EGFR Human)
   

   Tags

   3C cleavge site, protein C peptide tag, his6, SBP…

   Expression

   Mammalian

   Mutation

   EGFR amino acids 1−998

   Promoter

   CVM

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

6. KN801: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-VPR

   Plasmid

   #121829

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/VPR (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB

   Plasmid

   #121830

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/KRAB (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. KO001: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-p300core

   Plasmid

   #121832

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/p300core (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-ARL1

   Plasmid

   #67316

   Purpose

   hArl1 with stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL1 (ARL1 Human)
   

   Use

   Gateway entry vector

   Expression

   Bacterial

   Mutation

   bp 231 C to T; silent mutation

   Promoter

   None

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA6-E2F4CA-Myc

    Plasmid

    #80428

    Purpose

    Mammalian expression of constitutively active chick E2F4

    Depositor

    José María Frade

    Article

    Morillo et al Mol Cell Biol. 2012 Jul;32(14):2722-37.

    Insert

    E2F transcription factor 4 (E2F4 Chicken)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T261E, T263E

    Promoter

    CMV

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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