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pCin4-3xFLAG-MKL1 D444-630

Plasmid

#19840

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 D444-630 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 444-630

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11

Plasmid

#193752

Purpose

human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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AIRAP 11: PSMD14.pEYFP.V1

Plasmid

#21801

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD14 (Psmd14 Mouse)

Tags

YFP

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pGEM mouse dbx-1 [TJ#11]

Plasmid

#16269

Depositor

Thomas Jessell

Article

Pierani et al Cell. 1999 Jun 25. 97(7):903-15.

Insert

dbx1 3'UTR (Dbx1 Mouse)

Expression

Bacterial

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pKT-IDH1(R132H)-IRES-Katushka

Plasmid

#124257

Purpose

Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)

Expression

Mammalian

Mutation

Arginine 132 is mutated to Histidine. This mutati…

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-HsBIN1_tv8

Plasmid

#176012

Purpose

Mammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBH

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pN1_HsKIF1C(349-1103)-mNG

Plasmid

#253327

Purpose

mammalian expression of the stalk+tail domains of human KIF1C (aa 349-1103) tagged with mNG.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(349-1103) (KIF1C Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK211

Plasmid

#245159

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 11 (GTCACCAGT)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pKG0893_pHarbor-LentiX1_backbone

Plasmid

#239704

Purpose

PaqCI Golden Gate assembly plasmid encoding the LentiX1 backbone

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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PAL-GFP

Plasmid

#221175

Purpose

Expresses the palmitolylation region of Arl13b and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13b (ARL13B )

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 10-428

Promoter

CMV promoter

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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Arl13b-RVEP-4A-GFP

Plasmid

#221161

Purpose

Expresses mammalian Arl13b with RVEP4A mutation and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

RVEP at 358-361 mutated to AAAA

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1580_ECOR31HamAB_CoPurification

Plasmid

#223371

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1464_pTet-ECOR31Ham_DELhamA_Mut

Plasmid

#223365

Purpose

Expression of ECOR31 Hachiman HamB (∆HamA) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamB (∆HamA)

Expression

Bacterial

Mutation

∆HamA

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1465_pTet-ECOR31Ham_DELhamB_Mut

Plasmid

#223366

Purpose

Expression of ECOR31 Hachiman HamA (∆HamB) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamA (∆HamB)

Expression

Bacterial

Mutation

∆HamB

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1467_pTet-ECOR31Ham_hamB_walkerB_Mut

Plasmid

#223367

Purpose

Expression of ECOR31 Hachiman mutant (HamB D431A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamB D431A)

Expression

Bacterial

Mutation

HamB D431A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1469_pTet-ECOR31Ham_hamA_E138A_K140A_Mut

Plasmid

#223368

Purpose

Expression of ECOR31 Hachiman mutant (HamA E138A, K140A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamA E138A, K140A)

Expression

Bacterial

Mutation

HamA E138A, K140A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1444_ECOR31HamA_purification

Plasmid

#223369

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman HamA

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman HamA

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1445_ECOR31HamB_purification

Plasmid

#223370

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman HamB

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman HamB

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pKG0618_pPV1-CMV

Plasmid

#239688

Purpose

BsaI Golden Gate asembly plasmid encoding the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

CMV promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKG1179_pPV1-EFS

Plasmid

#239690

Purpose

BsaI Golden Gate assembly plasmid encoding the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

EFS promoter

Use

Synthetic Biology

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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