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pDRM98 mKate2 Blast

Plasmid

#174720

Purpose

Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

mKate2
BSD

Use

Lentiviral

Tags

P2A linker and SV40 NLS with GGS linker

Expression

Mammalian

Promoter

MND and MND (off mKate-P2A)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-EB3mCherry_cmv-LifeActGFP

Plasmid

#133927

Purpose

Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EB3-mCherry and Lifeact-GFP (MAPRE3 Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

Plasmid

#113885

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-PYL1-sfGFP-PML

Plasmid

#167011

Purpose

expression of PYL1-SfGFP-PML, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

PML (PML Human)

Use

Lentiviral

Tags

GFP-PML

Expression

Mammalian

Available Since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

Plasmid

#105200

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(HC)-P2A-antiPD1(LC)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

Plasmid

#156440

Purpose

For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA1 (Stag1 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-GRAM1b

Plasmid

#211494

Purpose

Expression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cells

Depositor

Yasunori Saheki

Article

Naito et al Elife. 2019 Nov 14;8. pii: 51401. doi: 1

Insert

GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE6.3

Plasmid

#102916

Purpose

expresses ABE6.3 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE6.3

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLI

Plasmid

#201440

Purpose

Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Tags

HA

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMK425 (GAL-OsTIR1(F74A))

Plasmid

#140657

Purpose

GAL-OsTIR1(F74A)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74A)

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ23-NLS

Plasmid

#231891

Purpose

Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q23-NLS (HTT Human)

Tags

EGFP and SV40 NLS

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1_WT RAD23B

Plasmid

#201457

Purpose

Expresses human RAD23B with an N-terminal GST tag in E. coli

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

GST

Expression

Bacterial

Mutation

Coding sequence has been optimised for expression…

Promoter

tac promoter

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC_P2A_Hygro_Barcode

Plasmid

#120461

Purpose

Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC (MYC Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pHSN401

Plasmid

#50588

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

3×FLAG-NLS-zCas9-NLS
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG and NLS

Mutation

maize codon optimized

Promoter

2×35Sp and AtU6-26p

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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CoV2-N-S202R

Plasmid

#177950

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S202R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (S202R) (N )

Expression

Mammalian

Mutation

S202R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJH2685

Plasmid

#191372

Purpose

Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Tetanus Toxin Light Chain

Tags

wCherry

Expression

Worm

Promoter

Punc-4

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

Plasmid

#156445

Purpose

For transient expression of mouse RAD21 (SCC1) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse RAD21 (Rad21 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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INPP5D-eGFP WT

Plasmid

#161998

Purpose

Mammalian expression vector, containing a GFP tagged version of INPP5D

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP5D (INPP5D Human)

Tags

eGFP

Expression

Mammalian

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.9

Plasmid

#102918

Purpose

expresses ABE7.9 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.9

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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