We narrowed to 15,732 results for: LEA

1. pDY0739 pCAG-NLS-SacINT

   Plasmid

   #179114

   Purpose

   SacINT expression vector

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCAG-NLS-SacINT
   

   Use

   CRISPR

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hnEF Coff/Fon hChR2(H134R)-EYFP

   Plasmid

   #55649

   Purpose

   Cre-off/Flp-on ChR2-EYFP under the short Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre off/flp on chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   Short Ef1

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-MCU-EGFP

   Plasmid

   #181866

   Purpose

   Expresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

   Insert

   mitochondrial calcium uniporter (Mcu Mouse)
   

   Use

   AAV and Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

   Plasmid

   #208961

   Purpose

   Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-Hsp90 N(9-236)

   Plasmid

   #22481

   Depositor

   William Sessa

   Article

   Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

   Insert

   Hsp90 alpha N domain (HSP90AA1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAGT9569

   Plasmid

   #219431

   Purpose

   Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

   Depositor

   Tom Schreiber

   Article

   Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

   Insert

   LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
   

   Use

   CRISPR and Synthetic Biology; Moclo compatible le…

   Tags

   PapE-4LF2

   Expression

   Plant

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 AIF-mScarlet

   Plasmid

   #129576

   Purpose

   Expression of mScarlet fused AIF

   Depositor

   Stephen Tait

   Article

   Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

   Insert

   AIF (AIFM1 Human)
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

   Plasmid

   #55646

   Purpose

   Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre on/flp off chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDY0312 PhiBT Integrase

   Plasmid

   #179111

   Purpose

   PhiBT Integrase expression vector

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   PhiBT Integrase
   

   Use

   CRISPR

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRHR2-DuET

    Plasmid

    #213216

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CRHR2 (CRHR2 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TVBB C-term-mNeongreen-Blast

    Plasmid

    #169229

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen-P2A-Blast
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pclbw-opa1(isoform 7DeltaS1)-myc

    Plasmid

    #62846

    Purpose

    Mammalian Expression of myc-tagged Opa1 isoform 7 Delta S1

    Depositor

    David Chan

    Article

    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

    Insert

    mmOPA1isoform7 DeltaS1 - myc (Opa1 Mouse)
    

    Use

    Retroviral

    Tags

    9xMyc

    Expression

    Mammalian

    Promoter

    cmv-bactin

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

    Plasmid

    #208976

    Purpose

    Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    nSpCas9(H840)-BPNLS-P2A-GFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-hM₃O-IP

    Plasmid

    #46645

    Depositor

    Nobuaki Kikyo

    Article

    Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

    Insert

    hM₃O (POU5F1 Human)
    

    Use

    Retroviral

    Tags

    IRES Puro and human MyoD (aa 1-62)

    Expression

    Mammalian

    Mutation

    contains a fragment of human MyoD (aa1-62) fused …

    Available Since

    July 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-nEF Con/Foff hChR2(H134R)-EYFP

    Plasmid

    #55647

    Purpose

    Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp off chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    Short Ef1a

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 AIF-mClover

    Plasmid

    #129575

    Purpose

    Expression of mClover fused AIF

    Depositor

    Stephen Tait

    Article

    Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

    Insert

    AIF (AIFM1 Human)
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC13P-iCLHN

    Plasmid

    #66580

    Purpose

    CLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENs

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Nanoluc-HaloTag
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-shMcu-2

    Plasmid

    #181867

    Purpose

    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    hrGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDB-His-MBP-mGSDMD

    Plasmid

    #123365

    Purpose

    Expression of full-length mouse GSDMD in E. coli

    Depositor

    Hao Wu

    Article

    Orning et al Science. 2018 Nov 30;362(6418):1064-1069

    Insert

    GSDMD (Gsdmd Mouse)
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKM-U6-reRNA-hsHEKsite_1_3

    Plasmid

    #205638

    Purpose

    reRNA guide targeting HEKsite_1_3

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    reRNA-HEKsite_1_3 (EPHA3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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