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pKG2019_pPV1-EF1a

Plasmid

#239687

Purpose

BsaI Golden Gate assembly plasmid encoding the EF1a promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

EF1a promoter

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SV40-puro-EF1a-HA-NLGN1dAChE-bc

Plasmid

#220437

Purpose

Expresses HA-tagged human NLGN1 with K578A/V579A substitutions

Depositor

Paul Blainey

Article

Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

Insert

Neuroligin-1 (NLGN1 Human)

Use

Lentiviral

Tags

HA-tag

Expression

Mammalian

Mutation

K578A and V579A substitutions

Promoter

EF1a

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-2H11

Plasmid

#239086

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of EVA1B

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Leucine 30 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-pos-1F02

Plasmid

#239087

Purpose

Positive control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of AMPE

Tags

MBP and ToxR

Expression

Bacterial

Mutation

deleted amino acids 19-22, and changed Leucine 35…

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-pos-2H07

Plasmid

#239088

Purpose

Positive control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of FND10

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Valine 184 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-2E11

Plasmid

#239084

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of LRIG3

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Valine 827 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-2F12

Plasmid

#239085

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of LRIG3

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Valine 815 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB027

Plasmid

#234636

Purpose

pET-28a(+) based plasmid for expression of H16_B1102 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1102 (H16_RS24270 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB028

Plasmid

#234637

Purpose

pET-28a(+) based plasmid for expression of H16_B1148 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1148 (H16_RS24495 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB029

Plasmid

#234638

Purpose

pET-28a(+) based plasmid for expression of H16_B1264 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1264 (H16_RS25065 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB030

Plasmid

#234639

Purpose

pET-28a(+) based plasmid for expression of H16_B1335 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1335 (H16_RS25400 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB024

Plasmid

#234633

Purpose

pET-28a(+) based plasmid for expression of H16_A0285 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A0285 (H16_RS01400 Cupriavidus necator H16)

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB026

Plasmid

#234635

Purpose

pET-28a(+) based plasmid for expression of H16_A3514 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A3514 (H16_RS17530 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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CT751

Plasmid

#233657

Purpose

Dox-inducible VP64-MyoD

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

VP64-MyoD

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT754

Plasmid

#233659

Purpose

FLEx switch with tTA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

tTA FLEx switch

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ABCB10-eYFP

Plasmid

#206038

Purpose

Express YFP in mitochondria

Depositor

Lufang Zhou

Article

Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

Insert

EYFP

Tags

ABCB10 mitochondrial leading sequence

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAM-CaMKII-EGFP-T2A-FlipO

Plasmid

#232791

Purpose

AAV2 vector containing EGFP and FlipO sequences separated by T2A peptide under the control of CaMKIIA promoter.

Depositor

Peer Wulff

Article

Baccini et al bioRxiv 2024.08.13.606964

Insert

CaMKII-EGFP-T2A-FlipO

Use

AAV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-roxSTOProx-NaChBac-TdTomato

Plasmid

#236253

Purpose

Dre dependent NaChBac-TdTomato expression

Depositor

Janelia Viral Tools

Article

Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

Insert

NaChBac-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Promoter

hSyn

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAM-FLEX-hM4D(Gi)-mCherry

Plasmid

#232782

Purpose

AAV2 vector containing Gi-coupled hM4D DREADD fused with mCherry inside a double floxed (FLEX) cassette under the control of CBA promoter.

Depositor

Peer Wulff

Article

Baccini et al bioRxiv 2024.08.13.606964

Insert

hM4D(Gi)-mCherry

Use

AAV

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-SP5

Plasmid

#232653

Purpose

transient expresses SP5 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

SP5 (SP5 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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