Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,071 results for: sgRNA

Showing: 7361 - 7380 of 9071 results

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pSEVA47_dCas9-GFP_NOT_i1

Plasmid

#231416

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

Depositor

Manish Kushwaha

Publication

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP

Use

Synthetic Biology

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-a11helixDel-sgEIF3D

Plasmid

#222135

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Publication

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

T317E, N320E, H321A

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-CtermDel-sgEIF3D

Plasmid

#222125

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Publication

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

F527 truncation

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-a5helixDel-sgEIF3D

Plasmid

#222133

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Publication

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

D249Q, V262I, Y263A

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-S528N-S529N-sgEIF3D

Plasmid

#222129

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Publication

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528N, S529N

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuideB3GNT5-neo

Plasmid

#220867

Purpose

encodes CRISPR sgRNA targeting human B3GNT5

Depositor

Jennifer Kohler

Publication

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

B3GNT5 (B3GNT5 Human)

Use

Lentiviral

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuideSLC35C1-neo

Plasmid

#220868

Purpose

encodes CRISPR sgRNA targeting human SLC3C1

Depositor

Jennifer Kohler

Publication

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

SLC35C1 (SLC35C1 Human)

Use

Lentiviral

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUCSh2-mRFP

Plasmid

#170982

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Publication

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199487

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199489

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

Plasmid

#199491

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

Plasmid

#199492

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKozak_mTagBFP2_synCoTC

Plasmid

#199493

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mTagBFP2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKozak_mTagBFP2-T2A-sGFP1-10_synCoTC

Plasmid

#199494

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pKozak_mTagBFP2-T2A-sGFP11x7_synCoTC

Plasmid

#199495

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

Plasmid

#199496

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mTagBFP2

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199497

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

Plasmid

#199498

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pSA_1_mTagBFP2-T2A-sGFP1-10_synCoTC

Plasmid

#199476

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pSA_2_mTagBFP2-T2A-sGFP1-10_synCoTC

Plasmid

#199477

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Publication

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge

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