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We narrowed to 922 results for: cas12a

Showing: 721 - 740 of 922 results

  1. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

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  2. pTF-lacZ-rfp

    Plasmid
    #153037
    Purpose
    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153037-plasmid-map-sequence-id-318855

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  3. pTF-ahpC-rfp

    Plasmid
    #153035
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153035-plasmid-map-sequence-id-318838

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  4. pTF-lacZ

    Plasmid
    #153036
    Purpose
    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153036-plasmid-map-sequence-id-318836

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  5. pAC1430

    Plasmid
    #127981
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127981-plasmid-map-sequence-id-254494

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  6. pUDP293

    Plasmid
    #204228
    Purpose
    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA
    Depositor
    Jean-Marc Daran
    Article
    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d
    Inserts
    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    Use
    Synthetic Biology
    Tags
    NLS
    Expression
    Mutation
    codon optimized for S. cerevisiae
    Promoter
    Saccharomyces cerevisiae PGK1 and Saccharomyces c…
    Available Since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    204228-plasmid-map-sequence-id-408527

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  7. pAC1749

    Plasmid
    #127983
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127983-plasmid-map-sequence-id-254502

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  8. pAC1750

    Plasmid
    #127984
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127984-plasmid-map-sequence-id-254510

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  9. pC1.530

    Plasmid
    #205441
    Purpose
    Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.
    Depositor
    Alistair McCormick
    Article
    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:
    Insert
    RepB gRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    205441-plasmid-map-sequence-id-408759

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  10. pAC1748

    Plasmid
    #127982
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    127982-plasmid-map-sequence-id-254497

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  14. gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217342
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available Since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    217342-plasmid-map-sequence-id-429351

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Showing: 721 - 740 of 922 results