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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,104 results for: gibson assembly

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Showing: 721 - 740 of 1104 results

  1. pBN202

    Plasmid
    #167950
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167950-plasmid-map-sequence-id-344771

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  2. pBN093

    Plasmid
    #167951
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, delete motif 3
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167951-plasmid-map-sequence-id-344776

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  7. pST39-Mega'

    Plasmid
    #216877
    Purpose
    Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.
    Depositor
    George Lu
    Article
    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:
    Insert
    Derived from Mega, and GvpS and GvpK overlap has been resolved
    Expression
    Bacterial
    Available Since
    Sept. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    216877-plasmid-map-sequence-id-445024

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  11. AP1298‐1

    Plasmid
    #87248
    Purpose
    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
    Depositor
    Geraldine Seydoux
    Article
    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12
    Insert
    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
    Available Since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87248-plasmid-map-sequence-id-173231

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  13. SpyTag003-OspC

    Plasmid
    #159998
    Purpose
    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells
    Depositor
    Mark Howarth
    Article
    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:
    Insert
    SpyTag003-OspC (20-209) C129S
    Tags
    His6-tag and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    C129S mutation improves expression level.
    Promoter
    T7
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159998-plasmid-map-sequence-id-316165

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  14. U4m

    Plasmid
    #121017
    Purpose
    MoClo golden gate assembly BC part for UTR1 (very strong RBS). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UTR1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121017-plasmid-map-sequence-id-236179

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Showing: 721 - 740 of 1104 results