We narrowed to 14,223 results for: Cas9
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Plasmid#136409PurposeDonor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.DepositorTypeEmpty backboneUseCRISPRMutationBbsI restriction site in the PDGFB sequence (Glu1…PromoterU6, PDKAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by
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VRQR-HF1 variant
Plasmid#138563PurposeExpresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSDDepositorInsertVRQR-HF1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
QQR1 variant
Plasmid#138564PurposeExpresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSDDepositorInsertQQR1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationG1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMini-CMV-NLS-dead R-IscB_ADAR2dd-NES_T2A_mCherry (W98X)_U6-ωRNA
Plasmid#246432PurposeAll-in-one plasmid. Expresses R-IscB_ADAR2dd in mammalian cells for A-to-I RNA editing. Inactive mCherry included to assess A-to-I editing. Spacer included can direct mCherry fluorescence recovery.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsO.gue IscB with dead mutations (D60A, H269A) and delta-TID, fused to human ADAR2 deaminase domain (ADARB1 Human, Synthetic, human gut metagenome)
mCherry
ωRNA
TagsHIV NES, SGGSSGGSSGSETPGTSESATPESSGGSSGGS linker …ExpressionMammalianMutationR-IscB: changed Aspartic Acid 60 to Alanine, chan…PromoterCMV and U6Available sinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pADH99Cau
Plasmid#244807PurposeModified plasmid from pADH99 to perform HIS-FLP CRISPR in Candidozyma aurisDepositorInsertCauHIS1_US
UseCRISPRTagsC. albicans MAL2 promoter, C. albicans codon opti…ExpressionYeastAvailable sinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_S34F_U2AF1
Plasmid#176592PurposeAdenoviral construct to change S to F at 34th amino acid (S34F) in human U2AF1DepositorInsertU2AF1 (U2AF1 Human)
UseAAVAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDM052
Plasmid#216814PurposeAspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.DepositorInsertmNeonGreen
TagsVinnyLinkerExpressionBacterialMutationAspergillus nidulans codon-adjustedAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTZ tRNA_gRNA empty scaffold
Plasmid#188450PurposeExpression of gRNAs using a human tRNA promoterDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromotertRNA glutamineAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgCRY1
Plasmid#176511PurposeExpresses sgRNA in mammalian cellsDepositorAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-2x
Plasmid#164414PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXZX352
Plasmid#169456PurposeMATA fragment in pTOPO vectorDepositorInsertMata locus
ExpressionBacterialPromoterlacAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT024
Plasmid#171636PurposePlasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2DepositorInsertsspyCas9 sgRNA-BFP
mTagBFP2
UseCRISPR and LentiviralExpressionMammalianPromoterCAG and U6Available sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Blat-BSD
Plasmid#163791PurposeThe plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.DepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
p33Fn
Plasmid#158155PurposeExpression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRExpressionPlantMutationRice codon optimizedAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICH67131
Plasmid#153223PurposeLevel 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminatorDepositorInsertNos promoter, NPTII, Nos terminator
UseSynthetic BiologyAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg1+sg2+sg3
Plasmid#113969PurposeTriple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTCDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsg1+sg2+sg3
ExpressionMammalianPromoterU6Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GSG1l-GFP KI
Plasmid#131492PurposeEndogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA and GFP donor (Gsg1l Rat)
ExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORANGE CACNG8-GFP KI #1
Plasmid#131473PurposeEndogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA and GFP donor (Cacng8 Rat)
ExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA
Plasmid#124546PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertWT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
ExpressionMammalianMutationWT, WTPromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124543PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
ExpressionMammalianMutationd11-41 C55G, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only